A comprehensive SAGE database for the analysis of gammadelta T cells

International Immunology
Jill C GraffMark A Jutila

Abstract

Gammadelta T cells have been conserved since the adaptive immune system arose, yet their importance is still unclear. In an attempt to compensate for the lack of a broad knowledge base of gammadelta T cells across species, global analyses of gammadelta T cell transcriptomes have been performed using serial analysis of gene expression (SAGE). Twelve new SAGE libraries were generated from the following bovine lymphocyte populations: magnetic bead-sorted blood gammadelta T cells, spleen gammadelta T cells and enriched spleen alphabeta T cells from a single calf, both rested and Con A/IL2 stimulated, and flow cytometry-sorted blood gammadelta and alphabeta T cells each either rested, Con A/IL2, or phorbol 12 myristate 13-acetate/ionomycin stimulated. These new libraries complement two earlier SAGE libraries of circulating gammadelta T cell subsets. These databases were analyzed using new web-based bioinformatic tools, which allow the user to rapidly compare gene expression patterns within these and other SAGE and standard expressed sequence tag libraries generated from different cell types and different species. These analyses revealed striking differences between blood and spleen gammadelta T cells and how these cells respond to m...Continue Reading

References

Jan 1, 1991·Immunology Today·W R Hein, C R Mackay
Jan 1, 1985·Journal of Cell Science. Supplement·J C MetcalfeJ P Moore
Jan 1, 1974·Annals of the New York Academy of Sciences·G N ReekeG M Edelman
Oct 20, 1995·Science·V E VelculescuK W Kinzler
Sep 26, 1997·Nature·K PolyakB Vogelstein
Aug 15, 1998·Critical Reviews in Immunology·A Salerno, F Dieli
Nov 26, 1999·Nucleic Acids Research·D G PetersA M Brufsky
Jul 26, 2000·Methods in Molecular Biology·J Powell
Jun 21, 2001·Molecular Cell Biology Research Communications : MCBRC·A J Darmon, P S Jat
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·A M FahrerY H Chien
Oct 9, 2001·Bioinformatics·J Colinge, G Feger
May 1, 2002·Nature Biotechnology·Saurabh SahaVictor E Velculescu
Jun 18, 2002·The EMBO Journal·Sheng WangDouglas V Faller
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole MeissnerMark A Jutila
Aug 2, 2003·The Journal of Biological Chemistry·Mohammad IshaqVen Natarajan
Sep 23, 2003·Nature Immunology·Daniel J PenningtonAdrian C Hayday
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jodi F HedgesMark A Jutila
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annemiek B van SprielMark D Wright
Sep 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sumito OgawaChristopher K Glass
Mar 31, 2005·Veterinary Immunology and Immunopathology·Mingqi DengMitchell S Abrahamsen

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Citations

May 16, 2008·Journal of Leukocyte Biology·Sailasree NemaliMark T Quinn
Mar 19, 2014·Veterinary Immunology and Immunopathology·Jodi L McGillW Ray Waters
Mar 13, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Takahisa Yamada
Mar 19, 2008·Animal Health Research Reviews·Mark A JutilaJodi F Hedges

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