A comprehensive strategy for identifying long-distance mobile peptides in xylem sap

The Plant Journal : for Cell and Molecular Biology
Satoru OkamotoYoshikatsu Matsubayashi

Abstract

There is a growing awareness that secreted pemediate organ-to-organ communication in higher plants. Xylem sap peptidomics is an effective but challenging approach for identifying long-distance mobile peptides. In this study we developed a simple, gel-free purification system that combines o-chlorophenol extraction with HPLC separation. Using this system, we successfully identified seven oligopeptides from soybean xylem sap exudate that had one or more post-transcriptional modifications: glycosylation, sulfation and/or hydroxylation. RNA sequencing and quantitative PCR analyses showed that the peptide-encoding genes are expressed in multiple tissues. We further analyzed the long-distance translocation of four of the seven peptides using gene-encoding peptides with single amino acid substitutions, and identified these four peptides as potential root-to-shoot mobile oligopeptides. Promoter-GUS analysis showed that all four peptide-encoding genes were expressed in the inner tissues of the root endodermis. Moreover, we found that some of these peptide-encoding genes responded to biotic and/or abiotic factors. These results indicate that our purification system provides a comprehensive approach for effectively identifying endogenous ...Continue Reading

References

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y Matsubayashi, Y Sakagami
Nov 27, 1999·Science·C R SchopferJ B Nasrallah
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TakayamaA Isogai
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PearceC A Ryan
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Hirotaka Kumagai, Hiroshi Kouchi
Jun 23, 2005·BMC Plant Biology·Julia KehrPatrick Giavalisco
Apr 11, 2006·Journal of Proteome Research·Sophie AlvarezDaniel P Schachtman
Jun 1, 1991·Plant Physiology·C L Biles, F B Abeles
Aug 12, 2006·Science·Tatsuhiko KondoYouji Sakagami
Jul 20, 2007·Genes & Development·Kenta HaraTatsuo Kakimoto
Aug 19, 2007·Journal of Proteome Research·Michael A DjordjevicPeter M Gresshoff
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yukari AmanoYoshikatsu Matsubayashi
Mar 5, 2008·The Plant Journal : for Cell and Molecular Biology·Kentaro OhyamaYoshikatsu Matsubayashi
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuki HirakawaHiroo Fukuda
Dec 17, 2008·Plant & Cell Physiology·Satoru OkamotoMasayoshi Kawaguchi
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 27, 2009·Phytochemistry·Nicole J Dafoe, C Peter Constabel
Jun 16, 2009·Nature Chemical Biology·Kentaro OhyamaYoshikatsu Matsubayashi
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ryota KomoriYoshikatsu Matsubayashi
Nov 20, 2009·Plant & Cell Physiology·Jed A ChristiansonIain W Wilson
Jan 16, 2010·Nature·Jeremy SchmutzScott A Jackson
Jan 14, 2012·Genes & Development·Jin Suk LeeKeiko U Torii
Feb 4, 2012·The Arabidopsis Book·Yoshikatsu Matsubayashi
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sadaf NaseerNiko Geldner
Feb 13, 2013·The New Phytologist·Colin G N Turnbull, Rosa M Lopez-Cobollo
Jul 9, 2013·Current Opinion in Plant Biology·L A C J Voesenek, J Bailey-Serres
Aug 13, 2013·Nature Communications·Satoru OkamotoMasayoshi Kawaguchi
Nov 22, 2013·Journal of Experimental Botany·Nijat IminMichael A Djordjevic
Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Takashi SoyanoMasayoshi Kawaguchi

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Citations

Jun 1, 2016·Frontiers in Plant Science·Philip CarellaRobin K Cameron
Aug 24, 2016·Current Opinion in Plant Biology·Satoru OkamotoYoshikatsu Matsubayashi
Jun 14, 2016·Journal of Plant Research·Hanna NishidaMasayoshi Kawaguchi
Oct 29, 2017·Molecular & Cellular Proteomics : MCP·Neha PatelMichael A Djordjevic
May 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kentaro NakaminamiKousuke Hanada
May 31, 2017·The New Phytologist·Rory N PruittPamela C Ronald
Jul 31, 2018·Plant & Cell Physiology·Takatoshi Kiba
Oct 24, 2018·Journal of Experimental Botany·Emiko YoroMasayoshi Kawaguchi
May 6, 2019·Journal of Experimental Botany·Kelly ChapmanMichael A Djordjevic
Jun 25, 2019·Journal of Experimental Botany·Christine Kaufmann, Margret Sauter
Sep 30, 2017·Plant & Cell Physiology·Hiroki Tsutsui, Michitaka Notaguchi
Sep 4, 2019·Current Protocols in Plant Biology·Clarissa BoschieroWolf-Rüdiger Scheible
Aug 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sayan ChakrabortyGuozhou Xu
Nov 30, 2020·The Plant Journal : for Cell and Molecular Biology·Takuya YoshidaFuminori Takahashi
Jun 6, 2017·Current Opinion in Plant Biology·Thomas C de BangHideki Takahashi
Jun 29, 2021·Plant Biotechnology·Na YuanShinichiro Sawa
Jun 2, 2021·Journal of Experimental Botany·Han-Chung LeeMichael A Djordjevic

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