A comprehensive study of calcific aortic stenosis: from rabbit to human samples

Disease Models & Mechanisms
Laura Mourino-AlvarezMaria G Barderas

Abstract

The global incidence of calcific aortic stenosis (CAS) is increasing owing, in part, to a growing elderly population. The condition poses a great challenge to public health, because of the multiple comorbidities of these older patients. Using a rabbit model of CAS, we sought to characterize protein alterations associated with calcified valve tissue that can be ultimately measured in plasma as non-invasive biomarkers of CAS. Aortic valves from healthy and mild stenotic rabbits were analyzed by two-dimensional difference gel electrophoresis, and selected reaction monitoring was used to directly measure the differentially expressed proteins in plasma from the same rabbits to corroborate their potential as diagnostic indicators. Similar analyses were performed in plasma from human subjects, to examine the suitability of these diagnostic indicators for transfer to the clinical setting. Eight proteins were found to be differentially expressed in CAS tissue, but only three were also altered in plasma samples from rabbits and humans: transitional endoplasmic reticulum ATPase, tropomyosin α-1 chain and L-lactate dehydrogenase B chain. Results of receiver operating characteristic curves showed the discriminative power of the scores, whic...Continue Reading

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Citations

Mar 29, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Melissa LieuWalter J Koch
Nov 15, 2020·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Nelson Ivan CupitraRaul Narvaez-Sanchez
Jun 29, 2021·Frontiers in Cardiovascular Medicine·Ishita TandonKartik Balachandran

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Methods Mentioned

BETA
electrophoresis
biopsies
PCA

Software Mentioned

DeCyder Differential Analysis Software
DeCyder
ImageJ
GPS Explorer
Mascot
Skyline
Analyst
SPSS

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