A computer image processing system for quantification of zebrafish behavior

Journal of Neuroscience Methods
Satoru KatoKayo Sugitani


The tropical zebrafish (Danio rerio) has frequently been used for investigating developmental biology. Here, we developed a computer image processing system for quantifying zebrafish behavior. We could acquire an image of zebrafish freely moving in an aquarium using a CCD camera through a graphic I/O board. To acquire the image of moving zebrafish in real time, we required high spatial (256 x 256 pixels) and temporal (10 frames/s) resolution. Such a high speed of data analysis was accomplished using a skipping search method. By using a small aquarium, trackings of newborn zebrafish could be traced. The velocity of adult zebrafish (7.2 cm/s) was far faster than that of newborn zebrafish (1.8 cm/s). Furthermore, by separation of occluded images of two fish, we could acquire images of the two zebrafish. They behaved as in a school in which one fish chased the other. The chasing was defined by the distance, angle and approach of the two fishes. The chasing ratio of pairs of zebrafish was 37%, whereas those of pairs of different fish were significantly reduced to less than 20%. The present image processing system is a very useful tool for quantitatively scoring the schooling behavior of multiple fish.


Feb 22, 2008·Neuroinformatics·Tianming LiuStephen T C Wong
Apr 18, 2013·Zebrafish·Allan V KalueffZebrafish Neuroscience Research Consortium
Jun 14, 2013·Zebrafish·Ralf MikutNadine Peyriéras
Oct 7, 2008·Briefings in Functional Genomics & Proteomics·Harold A Burgess, Michael Granato
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Toru MatsukawaSatoru Kato
Mar 17, 2011·PloS One·Jonathan CachatAllan V Kalueff
Jun 2, 2014·Nature Methods·Alfonso Pérez-EscuderoGonzalo G de Polavieja
Jul 20, 2011·Journal of Neuroscience Methods·Shiye ChenXiaoyin Xu
Sep 15, 2010·Progress in Neuro-psychopharmacology & Biological Psychiatry·Christine Buske, Robert Gerlai
Oct 9, 2014·Behavior Research Methods·Ruth DoladoJosé F Pertusa
Dec 21, 2005·Journal of Neuroscience Methods·Tianming LiuStephen T C Wong
Oct 11, 2005·Behavioural Brain Research·Earl T LarsonRichard H Melloni
Sep 3, 2013·Progress in Retinal and Eye Research·Satoru KatoKazuhiro Ogai
Mar 5, 2015·Computational Biology and Chemistry·Tsukasa FukunagaWataru Iwasaki
Dec 12, 2012·Journal of Neuroscience Methods·Shunren XiaWeiming Xia
Mar 28, 2006·Vision Research·Michael L RisnerAshley Moore
Jul 12, 2016·Journal of Neuroscience Methods·Shubham NemaYogesh Bhargava
Jan 11, 2017·Scientific Reports·Violet MwaffoMaurizio Porfiri
Apr 29, 2006·Lab Animal·Margarette SisonRobert Gerlai
Apr 19, 2007·Nature Reviews. Genetics·Graham J Lieschke, Peter D Currie

Related Concepts

Behavior, Animal
Entire Optic Nerve
Fish <Chondrichthyes>
Optical Image Reconstruction

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.