PMID: 9182990May 1, 1997Paper

A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae

European Journal of Biochemistry
I StansfieldMick F Tuite

Abstract

Genetic studies have indicated that the product of the yeast SUP45 gene encodes a component of the translational-termination machinery. In higher eukaryotes, genes similar to SUP45 encode eukaryote release factor 1 (eRF1), which has a stop-codon-dependent peptidyl-release activity. Using a conditional-lethal mutant allele of SUP45 (sup45-2) and a combination of in vivo and in vitro approaches, we demonstrate that the product of the SUP45 gene (Sup45p or eRF1) is a factor required for translation termination in yeast. A homologous in vitro assay based on suppressor-tRNA-mediated readthrough of stop codons is used to show that a translating lysate from a sup45-2 mutant strain exhibits a termination defect when heated for short periods to greater than the non-permissive temperature (37 degrees C). This defect can be complemented with a purified preparation of Sup45p (eRF1) expressed in Eschericha coli. The termination defect in this strain appears to be due to an inability of the Sup45p protein to bind the ribosome, resulting in vivo in a reduced ability of Sup45p to release nascent polypeptides from the ribosome at the non-permissive temperature. Cell-free translation lysates from the sup45-2 strain do not show a defect in sense-...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1990·Molecular Microbiology·W J CraigenC T Caskey
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Mar 20, 1986·Journal of Molecular Biology·D C EusticeF Sherman
Jul 11, 1986·Nucleic Acids Research·P Breining, W Piepersberg
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M F TuiteC S McLaughlin
May 1, 1994·Current Genetics·I Stansfield, M F Tuite
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·K ItoY Nakamura
Jan 1, 1996·Methods in Molecular Biology·A D HartleyM F Tuite

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Citations

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·I L DerkatchS W Liebman
Dec 12, 2012·Annual Review of Medicine·Stuart W PeltzAllan Jacobson
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Apr 19, 2016·Viruses·Heather A VincentNathaniel J Moorman
Feb 25, 2011·Seminars in Cell & Developmental Biology·Reed B WicknerAmy Kelly
Oct 5, 2007·Methods in Enzymology·Cheng WuMatthew S Sachs
Apr 25, 2006·Biochemical and Biophysical Research Communications·Satoshi KofujiToshiaki Katada
May 7, 2010·Nucleic Acids Research·Gloria H MerrittTobias von der Haar
Mar 16, 2019·Nucleic Acids Research·Christian BeißelHeike Krebber
May 7, 2008·Molecular and Cellular Biology·Victoria A DoroninaJeremy D Brown
Apr 27, 2010·The Journal of Biological Chemistry·Yoshifumi KobayashiJoseph Dougherty
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bijoyita RoyAllan Jacobson
Apr 10, 2019·Yeast·Douglas R LykeAnita L Manogaran
Apr 21, 2021·PLoS Genetics·Kotchaphorn MangkalaphibanAllan Jacobson

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