A conductive cell-imprinted substrate based on CNT-PDMS composite

Biotechnology and Applied Biochemistry
Hanie KavandShahin Bonakdar

Abstract

Cell function regulation is influenced by continuous biochemical and biophysical signal exchange within the body. Substrates with nano/micro-scaled topographies that mimic the physiological niche are widely applied for tissue engineering applications. As the cartilage niche is composed of several stimulating factors, a multifunctional substrate providing topographical features while having the capability of electrical stimulation is presented. Herein, we demonstrate a biocompatible and conductive chondrocyte cell-imprinted substrate using polydimethylsiloxane (PDMS) and carbon nanotubes (CNTs) as conductive fillers. Unlike the conventional silicon wafers or structural photoresist masters used for molding, cell surface topographical replication is challenging as biological cells showed extremely sensitive to chemical solvent residues during molding. The composite showed no significant difference compared with PDMS with regard to cytotoxicity, whereas an enhanced cell adhesion was observed on the conductive composite's surface. Integration of nanomaterials into the cell seeding scaffolds can make tissue regeneration process more efficient.

References

May 1, 1996·Clinical Orthopaedics and Related Research·M BrittbergL Peterson
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Jun 27, 2006·Biophysical Journal·Boris Reynaud, Thomas M Quinn
Nov 8, 2006·Multiple Sclerosis : Clinical and Laboratory Research·S SotgiuS Musumeci
Jan 5, 2007·Lab on a Chip·Michael W Toepke, David J Beebe
May 29, 2007·Biophysical Journal·Marco P E WengerPatrick Mesquida
Jun 20, 2007·Aesthetic Plastic Surgery·Fabrício Carvalho TorresMarcus Castro Ferreira
Jun 6, 2009·Angewandte Chemie·Christopher J BettingerJeffrey T Borenstein
Mar 2, 2010·Chemistry & Biology·Cécilia Ménard-MoyonAlberto Bianco
Sep 26, 2013·ACS Nano·Morteza MahmoudiWolfgang J Parak
Oct 30, 2013·Advanced Materials·Ju-Hyuck LeeSang-Woo Kim
Apr 23, 2014·Tissue Engineering. Part B, Reviews·Rodrigo A SomozaArnold I Caplan
Jun 26, 2014·Journal of Neural Engineering·Joong Hoon LeeSang-Hoon Lee
Aug 31, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Rebecca E WiluszFarshid Guilak
Feb 20, 2015·Nature Communications·Ovijit ChaudhuriDavid J Mooney
Jul 3, 2015·Scientific Reports·Andrea PavesiRoger D Kamm
Oct 11, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Carina PreinHauke Clausen-Schaumann
May 20, 2016·ACS Applied Materials & Interfaces·Shahin BonakdarPhilippe Renaud
Feb 6, 2017·Tissue Engineering. Part a·Bach Q LeJan de Boer
May 10, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lulu ZhouJoseph Lunec
Aug 2, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Niamh FahyMartin J Stoddart
Jan 5, 2018·Sports Medicine and Arthroscopy Review·Timothy M Simon, Douglas W Jackson
Jul 8, 2018·Nature Nanotechnology·Rodney S Ruoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.