A confocal and electron microscopic comparison of interferon beta-induced changes in vesicular stomatitis virus infection of neuroblastoma and nonneuronal cells.

DNA and Cell Biology
Paul M D'Agostino, Carol Shoshkes Reiss

Abstract

Vesicular stomatitis virus (VSV) replication is highly sensitive to interferon (IFN)-induced antiviral responses. Pretreatment of sensitive cultured cells with IFNbeta results in a 10(4)-fold reduction in the release of infectious VSV particles. However, differences exist between the mechanisms of reduced infectious particle titers in cell lines of neuroblastoma and nonneuronal lineage. In L929-fibroblast-derived cells, using immunofluorescence confocal microscopy, infection under control conditions reveals the accumulation of VSV matrix, phosphoprotein (P), and nucleocapsid (N) proteins over time, with induced cellular morphological changes indicative of cytopathic effects (CPEs). Upon observing L929 cells that had been pretreated with IFNbeta, neither detectable VSV proteins nor CPEs were seen, consistent with type I IFN antiviral protection. When using the same techniques to observe VSV infections of NB41A3 cells, a neuroblastoma cell line, aside from similar viral progression in the untreated control cells, IFNbeta-treated cells illustrated a severely attenuated VSV infection. Attenuated VSV progression was observed through detection of VSV matrix, P, and N proteins in isolated cells during the first 8 h of infection. Howev...Continue Reading

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Citations

Sep 17, 2011·DNA and Cell Biology·Sreeja SarojiniCarol Shoshkes Reiss
Oct 10, 2013·ISRN Allergy·Geeta Devi LeishangthemAmit Kumar Dinda

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Methods Mentioned

BETA
GTPase
electron microscopy
confocal microscopy
chemical fixation

Software Mentioned

AMT Advantage
Leica AF
Volocity

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