A conformational B-cell epitope on the C-terminal end of the extracellular part of human thyroid peroxidase.

The Journal of Biological Chemistry
Valérie EstienneJ Ruf

Abstract

To investigate the B-cell autoimmune epitopes on human thyroid peroxidase (TPO), we generated proteolytic peptides by enzymatic hydrolysis of TPO in nondenaturing and nonreducing conditions. The hydrolysate was chromatographed on a reverse phase column. We eluted a material immunoreactive with both a TPO monoclonal antibody recognizing a linear epitope (mAb47, amino acid 713-721) and TPO autoantibodies (aAb) from patients. The aAb immunoreactivity, but not that of mAb47, was lost after reduction. Western blots after electrophoresis without reduction showed that the aAb and mAb47 were immunoreactive with a 66-kDa band and that aAb identified a doublet at 20 kDa. For electrophoresis under reducing conditions, the 66-kDa band resolved into two peptides of 40 and 26 kDa, whereas the doublet at 20 kDa remained unchanged. None of these reduced peptides was immunoreactive with aAb, whereas the 40-kDa peptide was immunoreactive with mAb47. The 40-kDa peptide extends from amino acid 549 to 933 of TPO, and its last 192 amino acids overlap the autoimmune 20-kDa peptide. After iodine labeling, the 20-kDa peptide lost its immunoreactivity. We conclude that the C-terminal end of the extracellular part of TPO, which includes all the tyrosine ...Continue Reading

References

May 18, 1990·Cell·W G LaverS J Smith-Gill
Apr 1, 1989·The Journal of Clinical Endocrinology and Metabolism·Y OkamotoH Morii
Apr 1, 1989·The Journal of Clinical Endocrinology and Metabolism·N YokoyamaG G Klee
Nov 1, 1985·The Journal of Clinical Endocrinology and Metabolism·L PortmannL J DeGroot
Nov 1, 1987·Molecular Endocrinology·R P MagnussonB Rapoport
Aug 1, 1966·The Journal of Clinical Investigation·L W BravermanS H Ingbar
Jan 1, 1995·European Journal of Endocrinology·M TonaccheraM Ludgate
Dec 1, 1994·Endocrine Reviews·A P Weetman, A M McGregor
Mar 1, 1996·The Journal of Biological Chemistry·P L ArscottJ R Baker
Mar 1, 1995·Bio/technology·F Cordier-OchsenbeinF Russo-Marie

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Citations

Jul 24, 2002·Biochemical and Biophysical Research Communications·Stéphanie BlanchinJean Ruf
Jun 14, 2005·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Luigi MuratoriDiego Vergani
May 15, 2001·Thyroid : Official Journal of the American Thyroid Association·P PichurinS M McLachlan
May 1, 2001·Thyroid : Official Journal of the American Thyroid Association·Z XiongB Rapoport
Sep 8, 2007·Thyroid : Official Journal of the American Thyroid Association·Sandra M McLachlan, Basil Rapoport
Mar 17, 2005·Journal of Autoimmune Diseases·Damien BressonSylvie Péraldi-Roux
Aug 22, 2012·Arthritis Research & Therapy·Hui GengRikard Holmdahl
Dec 29, 2000·International Reviews of Immunology·S M McLachlan, B Rapoport
Oct 16, 1999·Autoimmunity·F Grennan JonesB Rees Smith
Aug 16, 2005·Archives of Biochemistry and Biophysics·Jean Ruf, Pierre Carayon
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yun MaDiego Vergani

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