A conjugated aptamer-gold nanoparticle fluorescent probe for highly sensitive detection of rHuEPO-α.

Sensors
Jiefang SunJianwei Xie

Abstract

We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.

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Citations

Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Seyed Mohammad TaghdisiKhalil Abnous
Jul 10, 2012·Current Opinion in Chemical Biology·Kristin B Cederquist, Shana O Kelley
May 6, 2015·International Journal of Pharmaceutics·Noor Mohammad DaneshSeyed Mohammad Taghdisi
Jul 25, 2014·Biosensors & Bioelectronics·Marimuthu CitartanThean-Hock Tang
Oct 11, 2016·Analytical and Bioanalytical Chemistry·Yesim Somay SelbesUgur Tamer
Jul 17, 2021·Biotechnology and Applied Biochemistry·Samin NooranianReza Kazemi Oskuee

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Methods Mentioned

BETA
fluorescent resonance energy transfer
transmission electron microscopy
FRET

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