A conserved cluster of homeodomain binding sites in the mouse Hoxa-4 intron functions in Drosophila embryos as an enhancer that is directly regulated by Ultrabithorax

Developmental Biology
T E Haerry, W J Gehring

Abstract

The evolutionary conservation of the homeodomains suggests that their in vivo DNA binding sites may also be conserved between vertebrates and invertebrates. The regulatory function of the mouse Hoxa-4 and Hoxb-4 introns were analyzed in Drosophila since they both contain a cluster of three homeodomain binding sites, the HB1 element, which was also found in the introns of other Hox genes ranging from fish to humans as well as in the Ultrabithorax (Ubx) and decapentaplegic (dpp) genes of Drosophila. The enhancer of the Hoxa-4 intron was found to respond to several homeobox genes activating a lacZ reporter gene in particular cells of the epidermis in Drosophila embryos. The enhancer activity was found to be similar to previously described autoregulatory elements of Deformed (Dfd), the Drosophila homolog of Hoxa-4, but additional expression was observed in more posterior segments activated by Ubx and repressed by abdominal-A (abd-A). Point mutations in the homeodomain binding sites in HB1 abolished the enhancer activity. A second site suppression experiment showed that UBX interacts directly with the HB1 element. When the HB1 element in the Hoxa-4 intron was replaced by that of the mesodermal enhancer of dpp, which was previously s...Continue Reading

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Citations

Jan 23, 1998·The Journal of Experimental Zoology·J L CarrF H Ruddle
Sep 16, 2008·Development Genes and Evolution·Juan Pascual-AnayaJordi Garcia-Fernàndez
Nov 5, 2008·Genome Research·Steven G KuntzBarbara J Wold
Jun 12, 2003·Genome Research·Simona SantiniAxel Meyer
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·C B KimF Ruddle
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·H G BeltingF H Ruddle
Oct 13, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vincenzo AmodioMaria Capovilla
Feb 11, 2010·Developmental Biology·Beena PunnamoottilSilke Rinkwitz
Dec 13, 2005·Nature Reviews. Genetics·Joseph C PearsonWilliam McGinnis
Apr 5, 2002·The Journal of Experimental Zoology·Steven Q IrvineFrank H Ruddle
Aug 26, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T PapenbrockA Awgulewitsch
Nov 21, 2000·Molecular Phylogenetics and Evolution·C H ChiuG P Wagner

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