A conserved α helix of Bcs1, a mitochondrial AAA chaperone, is required for the Respiratory Complex III maturation

Biochemical and Biophysical Research Communications
Rie SawamuraMasatoshi Esaki

Abstract

Bcs1 is a transmembrane chaperone in the mitochondrial inner membrane, and is required for the mitochondrial Respiratory Chain Complex III assembly. It has been shown that the highly-conserved C-terminal region of Bcs1 including the AAA ATPase domain in the matrix side is essential for the chaperone function. Here we describe the importance of the N-terminal short segment located in the intermembrane space in the Bcs1 function. Among the N-terminal 44 amino acid residues of yeast Bcs1, the first 37 residues are dispensable whereas a hydrophobic amino acid in the residue 38 is essential for integration of Rieske Iron-sulfur Protein into the premature Complex III from the mitochondrial matrix. Substitution of the residue 38 by a hydrophilic amino acid residue affects conformation of Bcs1 and interactions with other proteins. The evolutionarily-conserved short α helix of Bcs1 in the intermembrane space is an essential element for the chaperone function.

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Citations

Apr 18, 2018·Nature Cell Biology·Stefan StoldtStefan Jakobs
Jun 21, 2018·Journal of Biochemistry·Abhijit ChowdhuryMasatoshi Esaki
Jan 26, 2020·Applied Microbiology and Biotechnology·Zhenbiao LiHongfei Wang
Nov 2, 2019·Mitochondrion·Mario H Barros, Gavin P McStay

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