A conserved initiator element on the mammalian poly(ADP-ribose) polymerase-1 promoters, in combination with flanking core elements, is necessary to obtain high transcriptional activity

Biochimica Et Biophysica Acta
M A LanielS L Guérin

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) is a conserved nuclear protein present in nearly all eukaryotes. In mammalian cells, its abundant expression and its ability to specifically bind to DNA strand breaks make it an important enzyme in the rapid cellular response to DNA damage. Although the promoter regions of the three known mammalian PARP-1 genes, from human, rat and mouse, are different, they share common features, such as multiple GC-rich regions, lack of a functional TATA box, and presence of a putative initiator element. In this study, we analyzed the core promoter region of the rat PARP-1 gene, and show that it contains a functional initiator element surrounding the transcription start site. This core element lies within an approximately 40-base-pair region that is highly conserved in all three mammalian PARP-1 promoters. Furthermore, we show that other core elements located upstream and downstream of the PARP-1 initiator, including a functional Sp1 target site, synergize to regulate rat PARP-1 transcription. As the initiator region of all three PARP-1 gene promoters is highly conserved, their transcriptional regulation is likely achieved through similar mechanisms.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 26, 1992·Nature·M S Satoh, T Lindahl
Oct 23, 1990·Biochimica Et Biophysica Acta·C F CesaroneU Bertazzoni
Dec 12, 1990·European Journal of Biochemistry·Y YokoyamaY Yamamoto
Dec 1, 1990·Molecular and Cellular Biology·M C BlakeJ C Azizkhan
Sep 1, 1989·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J ThibodeauP Moreau
Nov 2, 1989·Biochimica Et Biophysica Acta·R McNerneyA Johnstone
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Colgan, J L Manley
Jan 1, 1994·Molecular and Cellular Biology·R JavaheryS T Smale
Dec 22, 1995·The Journal of Biological Chemistry·T A Ince, K W Scotto
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Oct 23, 1997·Biochemical and Biophysical Research Communications·A SalminenV Solovyan
Nov 21, 1997·Biochemical and Biophysical Research Communications·S L OeiN Tomilin
Jan 7, 1998·Molecular and Cellular Biology·J KaufmannR Müller
Jan 15, 1998·European Journal of Biochemistry·M J BergeronS L Guérin
Aug 24, 1999·The Biochemical Journal·D D'AmoursG G Poirier
Sep 25, 1999·The Journal of Biological Chemistry·L Q BensonD S Wechsler

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Citations

May 16, 2009·Nucleic Acids Research·Ganit YardenRivka Dikstein
Nov 14, 2014·The FEBS Journal·Kate Beishline, Jane Azizkhan-Clifford
Mar 4, 2005·American Journal of Physiology. Cell Physiology·Takuya KitamuraYoshimi Homma

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