A conserved organization of transcription during embryonic stem cell differentiation and in cells with high C value.

Molecular Biology of the Cell
I Faro-Trindade, Peter R Cook

Abstract

Although we have detailed information on the alterations occurring in steady-state levels of all cellular mRNAs during differentiation, we still know little about more global changes. Therefore, we investigated the numbers of molecules of RNA polymerase II that are active--and the way those molecules are organized--as two mouse cells (aneuploid F9 teratocarcinoma, and euploid and totipotent embryonic stem cells) differentiate into parietal endoderm. Quantitative immunoblotting shows the number of active molecules roughly halves. Transcription sites (detected by light and electron microscopy after allowing engaged polymerases to extend nascent transcripts in bromouridine-triphosphate) are uniformly distributed throughout the nucleoplasm. The numbers of such sites fall during differentiation as nuclei become smaller, but site density and diameter remain roughly constant. Similar site densities and diameters are found in salamander (amphibian) cells with 11-fold larger genomes, and in aneuploid HeLa cells. We conclude that active polymerases and their nascent transcripts are concentrated in a limited number of discrete nucleoplasmic sites or factories, and we speculate that the organization of transcription is conserved during bot...Continue Reading

References

Jun 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·C L MummeryS Shen
Jul 1, 1988·The Journal of Experimental Zoology·P Ferretti, J P Brockes
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E G BerstineB Ephrussi
Jul 1, 1980·The Histochemical Journal·K T Tokuyasu
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D C LoJ P Brockes
Aug 9, 1996·The Journal of Biological Chemistry·M E Dahmus
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·F A Brook, R L Gardner
Jan 1, 1997·Methods in Molecular Biology·E J Robertson
Nov 21, 1997·Journal of Molecular Biology·C StemmerS Muller
Aug 26, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F J Iborra, P R Cook
Oct 8, 1998·The Journal of Cell Biology·R AbranchesP J Shaw
Mar 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A PomboP R Cook
May 17, 2000·Molecular Reproduction and Development·D L Kelly, A Rizzino
Mar 20, 2001·Nature Reviews. Genetics·D L Hartl
Apr 28, 2001·Biological Reviews of the Cambridge Philosophical Society·T R Gregory
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Aug 20, 2002·Functional & Integrative Genomics·Thomas M Harris, Geoffrey Childs
Aug 28, 2003·Nature Structural Biology·Stephen Buratowski
Sep 16, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Wouter de Laat, Frank Grosveld
Oct 25, 2003·Journal of Cell Science·Peter R Cook
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jamie M SpergerJames A Thomson
Sep 14, 2004·Nature Genetics·Cameron S OsbornePeter Fraser
Jun 28, 2005·Trends in Genetics : TIG·Robert A MartienssenDerek B Goto
Sep 6, 2005·Science·Jean-Michel Claverie
Jan 10, 2006·Developmental Cell·Eran MeshorerTom Misteli
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I Cardoso, M J Saraiva
Jul 5, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michaël BonPeter R Cook

❮ Previous
Next ❯

Citations

Jul 20, 2007·Histochemistry and Cell Biology·Ana Pombo
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Miguel R BrancoAna Pombo
Apr 20, 2013·Chemical Reviews·Argyris Papantonis, Peter R Cook
Jun 10, 2009·Nature Reviews. Genetics·Heidi Sutherland, Wendy A Bickmore
Nov 7, 2007·Nature Structural & Molecular Biology·Tom SextonSusan M Gasser
Jan 11, 2013·Nucleic Acids Research·Joshua D LarkinDavide Marenduzzo
Feb 28, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Scott DavidsonJennifer A Mitchell
Feb 12, 2013·BMC Biophysics·Ana Z Canals-HamannFrancisco J Iborra
Dec 22, 2010·Journal of Cell Science·Virginia A SpencerMina J Bissell
Nov 3, 2011·Journal of Cell Science·Christopher H Eskiw, Peter Fraser
Oct 24, 2009·PloS One·Ronald Hancock, Yasmina Hadj-Sahraoui
Feb 26, 2010·Journal of Biomedicine & Biotechnology·Andrew DownesAlistair Elfick
Jul 21, 2015·Genes·Virlana M ShchukaJennifer A Mitchell
Sep 16, 2009·The Journal of Cell Biology·Peter R Cook, Davide Marenduzzo
Jan 31, 2012·Seminars in Cancer Biology·Binwei DengPeter R Cook
Feb 7, 2007·Trends in Genetics : TIG·Davide MarenduzzoPeter R Cook
Jan 2, 2007·Trends in Cell Biology·Miguel R Branco, Ana Pombo
Apr 4, 2008·Journal of Cellular Biochemistry·Cameron S Osborne, Christopher H Eskiw
Oct 24, 2007·Seminars in Cell & Developmental Biology·Tom SextonPeter Fraser
Oct 27, 2009·Journal of Molecular Biology·Peter R Cook
Mar 12, 2015·Nature Reviews. Molecular Cell Biology·Ana Pombo, Niall Dillon
Apr 25, 2017·Nature Structural & Molecular Biology·Mariano BarbieriAna Pombo
Oct 9, 2014·The Journal of Medical Humanities·Jean-Pierre Charpy
Nov 1, 2006·Biochemical Society Transactions·I Faro-Trindade, P R Cook
Jul 18, 2008·Biochemical Society Transactions·David R F CarterPeter R Cook
Dec 6, 2006·The Journal of Cell Biology·Davide MarenduzzoPeter R Cook
May 23, 2008·Journal of Cell Science·Christopher H EskiwPeter R Cook
Jan 12, 2007·Development·Hitoshi Niwa
Jan 11, 2012·Epigenetics & Chromatin·Petros KolovosArgyris Papantonis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.