A conserved SET domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast

The Journal of Biological Chemistry
Mahito SadaieJun-ichi Nakayama

Abstract

SET domain-containing methyltransferases post-translationally modify a variety of cellular proteins, such as histones, cytochrome c, ribulose-bisphosphate carboxylase/oxygenase, and ribosomal proteins. In the fission yeast Schizosaccharomyces pombe, at least 13 SET domain-containing proteins have been identified in the genome, four of which are involved in transcriptional regulation through their modification of histone tails. However, the roles played by the other SET domain proteins in cellular processes and their physiological substrates remain unresolved. We show here that S. pombe Set11, a SET domain-containing protein encoded by SPCC1223.04c, specifically modifies Rpl12 (ribosomal protein L12). Recombinant Set11 prepared from Escherichia coli had catalytic activity and methylated a 17-kDa polypeptide in cellular extracts of set11 mutant cells. The methylated protein was isolated by two-dimensional gel electrophoresis or by reverse-phase chromatography and was identified as Rpl12 by mass spectrometry. In vitro methylation experiments using wild-type and mutant Rpl12 proteins verified that Set11 modified recombinant Rpl12 and suggested that its potential target site was lysine 3. The methylation site modified by Set11 was a...Continue Reading

References

May 25, 1991·Nucleic Acids Research·Y ImaiM Terada
Nov 8, 1996·The Journal of Biological Chemistry·D F LouieN G Ahn
Mar 6, 1998·Cellular and Molecular Life Sciences : CMLS·T JenuweinG Reuter
May 3, 2000·The Journal of Biological Chemistry·B PolevodaF Sherman
Feb 22, 2002·The Journal of Biological Chemistry·Ming-Kai ChernMing F Tam
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sang-Won LeeRichard D Smith
Jun 18, 2002·Current Opinion in Cell Biology·Monika Lachner, Thomas Jenuwein
Aug 6, 2002·Nature Genetics·Juan MataJürg Bähler
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ken-ichi Noma, Shiv I S Grewal
Feb 11, 2003·Trends in Biochemical Sciences·Ronen Marmorstein
Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert L Houtz, Archie R Portis
Feb 12, 2004·Methods in Enzymology·Duane R PilchWilliam M Bonner
May 18, 2004·Genes & Development·Gunnar SchottaThomas Jenuwein
Oct 12, 2004·The Biochemical Journal·Rafal SwierczMark T Bedford
Nov 5, 2004·Nature·Sergei ChuikovDanny Reinberg
Dec 24, 2004·Molecular Biology of the Cell·Xu PengJianhua Liu
May 11, 2005·Protein Science : a Publication of the Protein Society·Yonghao YuJulie A Leary
Aug 13, 2005·The Journal of Biological Chemistry·Tanya R Porras-YakushiSteven Clarke
Oct 29, 2005·Nucleic Acids Research·Monica CuccureseConcetta Pietropaolo
Feb 16, 2006·Molecular and Cellular Biology·François BachandPamela A Silver
Sep 29, 2006·The Journal of Biological Chemistry·Tanya R Porras-YakushiSteven Clarke
Nov 17, 2006·Nature·Jing HuangShelley L Berger
Jul 6, 2007·Molecular Microbiology·Bogdan Polevoda, Fred Sherman

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Citations

May 21, 2010·Biochemistry·Kristofor J WebbSteven G Clarke
Aug 28, 2013·Journal of Proteome Research·Xiaoxia DaiYinsheng Wang
Jul 30, 2010·Nature·Christine E Schaner TooleyIan G Macara
Apr 11, 2009·The Journal of Biological Chemistry·Audrey PerreaultFrancois Bachand
May 7, 2010·The Journal of Biological Chemistry·Atsuko ShiraiJun-ichi Nakayama
Oct 30, 2008·The Journal of Biological Chemistry·Kristofor J WebbSteven G Clarke
Jan 20, 2011·Molecular and Cellular Biology·Kyoko Hiragami-HamadaJun-Ichi Nakayama
Jul 17, 2010·Biochemistry. Biokhimii︠a︡·K S EgorovaL V Olenina
Mar 1, 2012·Genes & Genetic Systems·Akira HatanakaMasaya Oki
Feb 4, 2014·Biochemical and Biophysical Research Communications·Daniel T LadrorLloyd M Smith
Jul 31, 2012·FEBS Letters·Qi Zhang, Wilfred A van der Donk
Jan 17, 2012·Proteomics·Melissa A ErceMarc R Wilkins
Jul 4, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuma KamataMasaya Oki
Mar 10, 2011·The Journal of Biological Chemistry·Erina KitanoJun-ichi Nakayama
Mar 21, 2018·The Journal of Biological Chemistry·Dai HatakeyamaTakashi Kuzuhara
Apr 10, 2014·Molecular Systems Biology·Sylvain LanouetteJean-François Couture
Aug 31, 2019·EMBO Reports·Eriko OyaJun-Ichi Nakayama
May 11, 2018·The Journal of Biological Chemistry·Steven G Clarke
Oct 10, 2018·Nature Ecology & Evolution·Yuichiro HaraShigehiro Kuraku
Apr 23, 2014·The Journal of Biological Chemistry·Qian CaiYinsheng Wang
Apr 7, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Damiaan E H F MeviusEric di Luccio

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