A conserved threonine residue in the juxtamembrane domain of the XA21 pattern recognition receptor is critical for kinase autophosphorylation and XA21-mediated immunity.

The Journal of Biological Chemistry
Xuewei ChenPamela C Ronald

Abstract

Despite the key role that pattern recognition receptors (PRRs) play in regulating immunity in plants and animals, the mechanism of activation of the associated non-arginine-aspartate (non-RD) kinases is unknown. The rice PRR XA21 recognizes the pathogen-associated molecular pattern, Ax21 (activator of XA21-mediated immunity). Here we show that the XA21 juxtamembrane (JM) domain is required for kinase autophosphorylation. Threonine 705 in the XA21 JM domain is essential for XA21 autophosphorylation in vitro and XA21-mediated innate immunity in vivo. The replacement of Thr(705) by an alanine or glutamic acid abolishes XA21 autophosphorylation and eliminates interactions between XA21 and four XA21-binding proteins in yeast and rice. Although threonine residues analogous to Thr(705) of XA21 are present in the JM domains of most RD and non-RD plant receptor-like kinases, this residue is not required for autophosphorylation of the Arabidopsis RD RLK BRI1 (brassinosteroid insensitive 1). The threonine 705 of XA21 is conserved only in the JM domains of plant RLKs but not in those of fly, human, or mouse suggesting distinct regulatory mechanisms. These results contribute to growing knowledge regarding the mechanism by which non-RD RLKs ...Continue Reading

References

Aug 1, 1996·Biochemical Society Transactions·A ShevchenkoM Mann
Apr 3, 2002·The Journal of Biological Chemistry·Guo-Zhen LiuWen-Yuan Song
Jun 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·R BrueggemanA Kleinhofs
Oct 9, 2002·Trends in Biochemical Sciences·Gerard ManningSucha Sudarsanam
Dec 10, 2002·Science·G ManningS Sudarsanam
Jan 1, 2003·Veterinary Immunology and Immunopathology·Dirk Werling, Thomas W Jungi
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Xinli SunQifa Zhang
Mar 1, 2005·Biochimie·Philippe GualJean-François Tanti
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·Mawsheng ChernPamela C Ronald
Jan 21, 2006·PLoS Pathogens·Christopher Dardick, Pamela Ronald
Mar 3, 2006·Genes & Development·Silke RobatzekThomas Boller
May 20, 2006·The Plant Journal : for Cell and Molecular Biology·Xuewei ChenLihuang Zhu
Jun 24, 2006·Biochemical and Biophysical Research Communications·Sami AifaSamuel P S Svensson
Nov 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sang-Won LeePamela C Ronald
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ayako MiyaNaoto Shibuya
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kristina W Thiel, Graham Carpenter
Dec 28, 2007·Nature Reviews. Genetics·Ki-Hong JungPamela C Ronald

❮ Previous
Next ❯

Citations

Oct 12, 2012·Protoplasma·Silke Robatzek, Lennart Wirthmueller
Jan 6, 2011·The Journal of Biological Chemistry·Dörte Klaus-HeisenJulie V Cullimore
Aug 21, 2013·Genome Génome / Conseil National De Recherches Canada·Baoju YangJorge Dubcovsky
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Aug 28, 2012·Annual Review of Phytopathology·Meritxell Antolín-LloveraMartin Parniske
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xuewei ChenPamela C Ronald
Aug 22, 2013·FEBS Letters·Sandip SamaddarMaitrayee DasGupta
Nov 26, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Meiyan LiuLiuling Yan
May 24, 2011·Trends in Plant Science·Xuewei Chen, Pamela C Ronald
Jan 11, 2011·Current Opinion in Microbiology·Cécile Segonzac, Cyril Zipfel
Nov 16, 2010·Plant Science : an International Journal of Experimental Plant Biology·Chang-Jin ParkPamela C Ronald
Aug 10, 2013·Current Opinion in Plant Biology·Yoji Kawano, Ko Shimamoto
Mar 22, 2015·Trends in Plant Science·Alberto P MachoCyril Zipfel
Apr 29, 2014·Molecular Cell·Alberto P Macho, Cyril Zipfel
Feb 17, 2017·Journal of Experimental Botany·Pawel Z KosentkaElena D Shpak
Feb 2, 2018·Plant Signaling & Behavior·Maruya SuzukiNaoto Shibuya
Sep 14, 2017·International Journal of Molecular Sciences·Shuhua HuangXiaofeng Wang
Jul 2, 2010·Cellular Microbiology·Chang-Jin ParkPamela C Ronald
Jul 31, 2021·Plant Communications·Tsung-Chi ChenPamela Ronald
Aug 18, 2021·Molecular Cell·Thomas A DeFalco, Cyril Zipfel
Sep 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kyle W BenderCyril Zipfel

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.