PMID: 9445384Mar 7, 1998Paper

A conserved tryptophan in pneumolysin is a determinant of the characteristics of channels formed by pneumolysin in cells and planar lipid bilayers

The Biochemical Journal
Y E KorchevTim J Mitchell

Abstract

Pneumolysin is one of the family of thiol-activatable, cytolytic toxins. Within these toxins the amino acid sequence Trp-Glu-Trp-Trp is conserved. Mutations made in this region of pneumolysin, residues 433-436 inclusive, did not affect cell binding or the formation of toxin oligomers in the target cell membrane. However, the mutations did affect haemolysis, leakage of low-molecular-mass metabolites from Lettre cells and the induction of conductance channels across planar lipid bilayers. Of eight modified pneumolysins examined, Trp-433-->Phe showed the smallest amount of haemolysis or leakage (less than 5% of wild type). Pneumolysin-induced leakage from Lettre cells was sensitive to inhibition by bivalent cations but the extent of inhibition varied depending on the modification. Leakage by the mutant Trp-433-->Phe was least sensitive to cation inhibition. The ion-conducting channels formed across planar lipid bilayers exhibit small (less than 30 pS), medium (30 pS-1 nS) and large (more than 1 nS) conductance steps. Small- and medium-sized channels were preferentially closed by bivalent cations. In contrast with wild-type toxin, which formed predominantly small channels, the modified toxin Trp-433-->Phe formed large channels that...Continue Reading

Citations

Jul 30, 2005·The Journal of Physiology·Maryline BeurgDidier Dulon
Feb 26, 2016·European Biophysics Journal : EBJ·Robert J C Gilbert, Andreas F-P Sonnen
Jul 24, 2013·Biophysical Chemistry·Marta MarchiorettoGregor Anderluh
Apr 16, 2015·American Journal of Respiratory Cell and Molecular Biology·Sarah StattCheng-Yuan Kao
Apr 27, 2011·Cellular Microbiology·Manuel R GonzalezF Gisou van der Goot
Jul 28, 2004·The Journal of General Physiology·George Shapovalov, Henry A Lester
Mar 29, 2000·The Journal of General Physiology·S Olsnes, P O Falnes
Jun 16, 2006·FEMS Microbiology Reviews·Samer Kayal, Alain Charbit
Feb 12, 2008·Biochemical and Biophysical Research Communications·Rana G El-RachkidyPeter W Andrew
Apr 27, 2005·Cell·Sarah J TilleyHelen R Saibil
Jan 15, 2014·Biophysical Journal·Lidia PrietoThemis Lazaridis
Nov 14, 1998·Journal of Molecular Biology·J RossjohnM W Parker
Oct 22, 2016·Human Vaccines & Immunotherapeutics·Philippe HermandFabrice Godfroid
Apr 10, 2015·Scientific Reports·Marjetka PodobnikGregor Anderluh
Sep 25, 2007·Audiology & Neuro-otology·Valérie Franco-VidalDidier Dulon
May 29, 2015·Cell Death & Disease·T J LaRoccaA J Ratner
Sep 16, 2004·The Journal of Membrane Biology·A A SobkoY N Antonenko
Jun 2, 2007·Biological & Pharmaceutical Bulletin·Kazuto Ohkura
Mar 8, 2006·The Journal of Biological Chemistry·Adam J RatnerJeffrey N Weiser
Apr 25, 2014·Open Biology·Andreas F-P SonnenRobert J C Gilbert
Nov 30, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Walter AdamsJohn M Leong
Apr 17, 2001·Molecular Membrane Biology·B BonevA Watts
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kathryn A MatthiasJeffrey N Weiser
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather ZwaferinkThomas Decker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.