A conserved two-component regulatory system, PidS/PidR, globally regulates pigmentation and virulence-related phenotypes of Burkholderia glumae.

Molecular Plant Pathology
Hari S KarkiJong Hyun Ham

Abstract

Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B. glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B. glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, the PidS/PidR TCRS was also required for the elicitation of the hypersensitive response on tobacco leaves, indicating the dependence of the hypersensitive response and pathogenicity (Hrp) type III secretion system of B. glumae on this regulatory factor. In addition, B. glumae mutants defective in the PidS/PidR TCRS showed less production of the phytotoxin, toxoflavin, and less virulence on rice panicles and onion bulbs relative to the parental strain, 411gr-6. The presence of highly homologous PidS and PidR ortholog...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Jun 13, 1997·Biochimica Et Biophysica Acta·C Hägerhäll
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·A CollmerJ R Alfano
Aug 26, 2000·Journal of Microbiological Methods·Y M Kwon, S C Ricke
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Derrick E FoutsAlan Collmer
Jul 31, 2004·Annual Review of Phytopathology·James R Alfano, Alan Collmer
Apr 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert P RyanJ Maxwell Dow
Jul 14, 2007·Trends in Microbiology·Beth M MoleSarah R Grant
Dec 14, 2007·Annual Review of Genetics·Michael T Laub, Mark Goulian
Apr 2, 2011·Molecular Plant Pathology·Jong Hyun HamMilton C Rush

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Citations

Dec 23, 2015·Environmental Microbiology·Lynn M NaughtonJ Maxwell Dow
Apr 15, 2020·Infection and Immunity·Matthew M Schaefers

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