A Consistent Orally-Infected Hamster Model for Enterovirus A71 Encephalomyelitis Demonstrates Squamous Lesions in the Paws, Skin and Oral Cavity Reminiscent of Hand-Foot-and-Mouth Disease

PloS One
Win Kyaw PhyuKum Thong Wong

Abstract

Enterovirus A71 (EV-A71) causes self-limiting, hand-foot-and-mouth disease (HFMD) that may rarely be complicated by encephalomyelitis. Person-to-person transmission is usually by fecal-oral or oral-oral routes. To study viral replication sites in the oral cavity and other tissues, and to gain further insights into virus shedding and neuropathogenesis, we developed a consistent, orally-infected, 2-week-old hamster model of HFMD and EV-A71 encephalomyelitis. Tissues from orally-infected, 2-week-old hamsters were studied by light microscopy, immunohistochemistry and in situ hybridization to detect viral antigens and RNA, respectively, and by virus titration. Hamsters developed the disease and died after 4-8 days post infection; LD50 was 25 CCID50. Macroscopic cutaneous lesions around the oral cavity and paws were observed. Squamous epithelium in the lip, oral cavity, paw, skin, and esophagus, showed multiple small inflammatory foci around squamous cells that demonstrated viral antigens/RNA. Neurons (brainstem, spinal cord, sensory ganglia), acinar cells (salivary gland, lacrimal gland), lymphoid cells (lymph node, spleen), and muscle fibres (skeletal, cardiac and smooth muscles), liver and gastric epithelium also showed varying am...Continue Reading

References

Dec 1, 1995·Virus Research·B A Brown, M A Pallansch
Dec 8, 1998·The Journal of Pediatrics·L C LumM Lambert
Sep 25, 1999·The New England Journal of Medicine·M HoS R Shih
Sep 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·K C ChowC C Lee
Mar 27, 2001·Emerging Infectious Diseases·W J ShiehS R Zaki
Apr 4, 2002·Scandinavian Journal of Infectious Diseases·Chung-Chen LiKuender D Yang
Jan 21, 2003·Emerging Infectious Diseases·Kwai Peng ChanAi Ee Ling
Jan 14, 2004·The Journal of General Virology·Yi-Chun ChenHuan-Yao Lei
Jan 15, 2004·JAMA : the Journal of the American Medical Association·Luan-Yin ChangTzou-Yien Lin
Aug 17, 2005·Journal of Virology·Shannon L Taylor, Jennifer F Moffat
Feb 6, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mong How OoiTom Solomon
Jan 26, 2008·Journal of Neuropathology and Experimental Neurology·Kum Thong WongKazuo Nagashima
Jun 4, 2008·Journal of Neuropathology and Experimental Neurology·Kien Chai OngKum Thong Wong
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ken FujiiSatoshi Koike
Oct 8, 2014·Journal of Neuropathology and Experimental Neurology·Soon Hao TanKum Thong Wong
Jun 4, 2015·Emerging Microbes & Infections·Xingdong YangLijuan Yuan

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Citations

Jul 1, 2016·Scientific Reports·Carla Bianca Luena VictorioKaw Bing Chua
Mar 23, 2017·Scientific Reports·Win Kyaw PhyuKum Thong Wong
Jul 1, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuan Teng HooiKum Thong Wong

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Methods Mentioned

BETA
light microscopy
PCR
transgenic

Software Mentioned

SPSS
MEGA

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