PMID: 3762222Aug 1, 1986Paper

A constant perfusion slice chamber for stable recording during the addition of drugs

Journal of Neuroscience Methods
R A Palovcik, M I Phillips

Abstract

A new design for a brain slice chamber is described. This chamber has the following features: slices are maintained in a stable condition for long-term (3-4 h) intracellular recording; drugs may be injected into the flowing perfusion medium in known concentrations without disturbing intracellular penetration; a wide range of concentrations may be used to test a single cell, highly repeatable results may be obtained; only minute amounts are required of substances to be tested, and the apparatus is easy to use and clean since all parts are removable. The chamber may also be easily modified to allow for the requirements of different experiments. The chamber has been used successfully to test the effects of Ang II, carbachol, insulin and gonadotropin releasing hormone.

References

Dec 1, 1979·Journal of Neuroscience Methods·H L HaasM Vosmansky
Jan 1, 1986·Neuroendocrinology·R A Palovcik, M I Phillips
Mar 1, 1983·Journal of Neuroscience Methods·J F Koerner, C W Cotman
Aug 20, 1984·Brain Research·R A PalovcikM K Raizada
Dec 10, 1984·Brain Research·R A Palovcik, M I Phillips
Dec 1, 1984·Journal of Neuroscience Methods·B A MacVicar
Aug 1, 1981·Journal of Neuroscience Methods·R A Nicoll, B E Alger
Jul 1, 1980·Brain Research Bulletin·T J Teyler

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Citations

Feb 1, 1988·Journal of Neuroscience Methods·P C Rinaldi, A M Brown
Jun 23, 2009·Neuropeptides·Peter Kovacs, Andras Hajnal
Aug 19, 2009·Annals of the New York Academy of Sciences·Andras HajnalPeter Kovacs
Nov 16, 2010·Journal of Neuroscience Methods·M R H Hill, S A Greenfield
Dec 24, 1997·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S J BlackbandM I Phillips
Jun 17, 2014·Scientific Reports·Yossi BuskilaJohn W Morley

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