A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production

PloS One
Neha MunjalSyed Shams Yazdani

Abstract

The production of biofuels from lignocellulosic biomass appears to be attractive and viable due to the abundance and availability of this biomass. The hydrolysis of this biomass, however, is challenging because of the complex lignocellulosic structure. The ability to produce hydrolytic cellulase enzymes in a cost-effective manner will certainly accelerate the process of making lignocellulosic ethanol production a commercial reality. These cellulases may need to be produced aerobically to generate large amounts of protein in a short time or anaerobically to produce biofuels from cellulose via consolidated bioprocessing. Therefore, it is important to identify a promoter that can constitutively drive the expression of cellulases under both aerobic and anaerobic conditions without the need for an inducer. Using lacZ as reporter gene, we analyzed the strength of the promoters of four genes, namely lacZ, gapA, ldhA and pflB, and found that the gapA promoter yielded the maximum expression of the β-galactosidase enzyme under both aerobic and anaerobic conditions. We further cloned the genes for two cellulolytic enzymes, β-1,4-endoglucanase and β-1,4-glucosidase, under the control of the gapA promoter, and we expressed these genes in Es...Continue Reading

References

Sep 1, 1989·FEMS Microbiology Reviews·D P Clark
Nov 1, 1988·Journal of Bacteriology·G Sawers, A Böck
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
May 1, 1981·Journal of General Microbiology·M C PascalJ Knappe
Mar 6, 1998·Trends in Biotechnology·G Hannig, S C Makrides
Jun 5, 1998·Journal of Industrial Microbiology & Biotechnology·L P YomanoL O Ingram
Nov 18, 1998·Current Opinion in Structural Biology·E A BayerY Shoham
Aug 24, 1999·Journal of Industrial Microbiology & Biotechnology·B Saha, R Bothast
Oct 9, 1999·Biotechnology Progress·L R LyndT U Gerngross
Oct 9, 1999·Biotechnology Progress·L O IngramS Zhou
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
Feb 9, 2005·The Journal of Biological Chemistry·Kirsty A SalmonRobert P Gunsalus
May 19, 2005·Biotechnology Advances·Joseph Shiloach, Rephael Fass
Sep 13, 2005·Current Opinion in Biotechnology·Lee R LyndMark Laser
Aug 15, 2006·Protein Expression and Purification·Wolfgang Peti, Rebecca Page
Feb 10, 2007·Science·Gregory Stephanopoulos
Feb 12, 2008·Current Protocols in Molecular Biology·S Tabor
Jun 9, 2009·Current Opinion in Biotechnology·Antoine MargeotFrédéric Monot
Aug 15, 2009·International Journal of Biological Sciences·Miranda MakiWensheng Qin
Sep 30, 2009·Journal of Molecular Biology·Haeyoung JeongJihyun F Kim
Nov 7, 2009·Methods in Enzymology·Sarah ZerbsFrank R Collart
May 29, 2010·Applied Microbiology and Biotechnology·Daniel C la GrangeWillem H van Zyl
Sep 11, 2010·Science·Jonathan M GalazkaJamie H D Cate
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Suk-Jin HaYong-Su Jin
Jun 7, 2011·Applied and Environmental Microbiology·Nidhi AdlakhaSyed Shams Yazdani
Nov 19, 2011·Biotechnology and Bioengineering·Daniel Klein-MarcuschamerHarvey W Blanch
Nov 6, 2012·Microbial Cell Factories·Neha MunjalSyed Shams Yazdani
Dec 4, 2012·Protein Expression and Purification·Shefali GuptaSyed Shams Yazdani

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Citations

Oct 21, 2015·Journal of Bioscience and Bioengineering·Rajni DubeyNaseem A Gaur
Jul 6, 2017·Journal of Industrial Microbiology & Biotechnology·Syed Bilal JilaniSyed Shams Yazdani
May 19, 2019·Folia Microbiologica·Kalpana SahooEnketeswara Subudhi
Nov 26, 2020·Scientific Reports·Victoria L BarlowYu-Hsuan Tsai
Jan 12, 2021·Journal, Genetic Engineering & Biotechnology·Bhuvan Shankar VadalaAnjali Apte-Deshpande
Jan 24, 2021·Molecular Biotechnology·Alberto Moura Mendes LopesRosana Goldbeck

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Methods Mentioned

BETA
PCR

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