A constitutive nitric oxide synthase modulates insulin secretion in the INS-1 cell line

Molecular and Cellular Endocrinology
P BeffyRené Gross

Abstract

We provide immunocytochemical evidence that the neuronal isoform of constitutive NO synthase (cNOS) is expressed in the rat insulinoma cell line INS-1. Furthermore, using N omega-nitro-L-arginine methyl ester (L-NAME), a pharmacological inhibitor of cNOS activity, we show that this enzyme is implicated in the modulation of insulin secretion in INS-1 cells. Indeed, in the presence of 2.8 mM glucose, L-NAME induced a specific and dose-dependent increase in insulin release, suggesting that cNOS exerts an inhibitory tone on basal insulin secretion. Moreover, L-arginine, the physiological substrate of cNOS, significantly reduced the marked enhancing effect of L-NAME on insulin release and to a lesser extent, at low concentrations, that of 10 mM KCl. L-NAME also potentiated the insulin secretion stimulated by 5.5 and 8.3 mM glucose, but in this case, its effect was not reduced by L-arginine. In conclusion, our data show that the neuronal isoform of cNOS exerts a negative modulation on insulin secretion in INS-1 cells, confirming the previous results obtained in the isolated perfused rat pancreas or pancreatic islets.

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Citations

Aug 28, 2012·Nitric Oxide : Biology and Chemistry·Ewa Gurgul-ConveySigurd Lenzen
Aug 19, 2006·Endocrinology·Veronique AttaliGil Leibowitz
Jan 23, 2021·Biomedicines·Carlos M González-CasimiroGermán Perdomo

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