A continuous fluorescence assay for phospholipase A2 with nontagged lipid

Analytical Biochemistry
Changkang HuangLuhua Lai

Abstract

Human nonpancreatic secreted phospholipase A2 (hnps PLA2) is considered to be an important drug target for antiinflammation therapy. We have established a new fluorescence assay by using 1-anilinonaphthalene-8-sulfonate (ANS) as an interfacial probe for hydrophobic environment detection. The fitted apparent k(cat)/K(m) of hnps PLA2 is 0.0181 +/- 0.0005 RFU/microMs. Tests on known synthesized inhibitor gave IC50 values similar to those from isotope-labeled assay. Because ANS is a commonly used probe for hydrophobic environment detection that needs no modification in the current assay, this strategy may be widely applicable for interfacial catalytic reactions.

References

Mar 25, 1992·Biochimica Et Biophysica Acta·D MeyuhasD Lichtenberg
Aug 10, 1995·Analytical Biochemistry·G V Richieri, A M Kleinfeld
Nov 20, 1995·Analytical Biochemistry·T BayburtM H Gelb
Jun 2, 1997·Biochimica Et Biophysica Acta·S K HanW Cho
Aug 26, 1998·Journal of Biochemical and Biophysical Methods·Y YaoC C Wang
Dec 11, 1999·Proceedings of the Association of American Physicians·C O Bingham, K F Austen
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Nov 18, 2000·Biochimica Et Biophysica Acta·S YedgarE Schnitzer
Feb 8, 2002·Archives of Biochemistry and Biophysics·Rogelio Morales, Marta S Fernández
Jul 5, 2003·Analytical Biochemistry·M JiménezM Pérez-Gilabert
Aug 18, 2005·Bioorganic & Medicinal Chemistry Letters·Ying LiuLu-Hua Lai

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Citations

Aug 21, 2013·Applied Biochemistry and Biotechnology·Shunchen Qiu, Luhua Lai
Mar 13, 2014·Chemical Biology & Drug Design·Shunchen QiuLuhua Lai
Dec 29, 2013·Biochemical and Biophysical Research Communications·Shunchen Qiu, Luhua Lai
Jun 11, 2014·Analytical Biochemistry·Hamse K VivekS Nanjunda Swamy
May 20, 2016·Journal of Lipid Research·Meddy El AlaouiAbdelkarim Abousalham
Nov 2, 2018·Fish Physiology and Biochemistry·Leire ArantzamendiMarisol Izquierdo

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