Oct 1, 1988

A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase

Analytical Biochemistry
M StioG Pinzauti

Abstract

A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corresponds to a delta E of 0.24 min-1, when the enzyme is assayed in a microsomal fraction. We also discuss the method's suitability for subcellular fractionation. No other continuous assay for this important enzymatic marker of the endoplasmic reticulum is currently available.

  • References1
  • Citations4

References

  • References1
  • Citations4

Citations

Mentioned in this Paper

Glucosephosphatase
Enzymes, antithrombotic
Aldose 1-epimerase
NADH
Subcellular Fractionation
Microsomes, Liver
Ampholytes
H6PD gene
Enzymes for Treatment of Wounds and Ulcers
Spectrophotometry, Ultraviolet

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