PMID: 3778910Dec 1, 1986Paper

A contribution of calmodulin to cellular deformability of calcium-loaded human erythrocytes

Biochimica Et Biophysica Acta
J MurakamiT Shiga

Abstract

The effect of intracellular calcium on the deformability of human erythrocytes was studied with a rheoscope, especially in relation to the dynamic structure of membrane cytoskeleton. The appropriate calcium-loading and calcium-depletion were performed to intact erythrocytes with A23187 in potassium buffer. The total calcium content was varied in the range of 0.25 to 3 times as much as normal content, without complete ATP depletion and shape change (the reduction of mean cell volume and the condensation of hemoglobin due to dehydration were avoided). Increasing the intracellular calcium content by about 1.5 times of normal, the deformability was distinctly decreased, while calcium depletion did not affect the deformability. Reduced deformability of the calcium-loaded erythrocytes was restored by the treatment with calmodulin inhibitors, W-7 or trifluoperazine. However, such an effect by calmodulin inhibitors was not detected on normal or calcium-depleted erythrocytes. In conclusion, the interaction between calcium-calmodulin complex and cytoskeletal proteins may affect the membrane stiffness which is regulated through the change of the cytoskeletal structure, and contributes to the deformability of erythrocytes.

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Citations

Sep 1, 1992·Biulleten' eksperimental'noĭ biologii i meditsiny·A Sh ByshevskiĭS V Solov'ev
Nov 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M KurataN S Agar
Oct 1, 1994·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Kurata, M Suzuki
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L N Katyukhin Matskevich YuA
Jun 17, 1998·Free Radical Biology & Medicine·J MojzisL Mirossay
Jan 1, 1990·Critical Reviews in Oncology/hematology·T ShigaK Kon
May 18, 1993·Biochemistry·C J WallisE F Wenegieme
Jan 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Melek Bor-KucukatayOguz K Baskurt
Jan 10, 2002·Biochimica Et Biophysica Acta·Barbara Del CarloMario Pellegrino
Nov 2, 1990·Biochimica Et Biophysica Acta·Y SuzukiN Maeda
Jan 18, 1989·Biochimica Et Biophysica Acta·V Bennett

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