PMID: 1204656Dec 15, 1975Paper

A contribution of nonhistone proteins to the conformation of chromatin

European Journal of Biochemistry
T Tashiro, M Kurokawa

Abstract

1. Changes in circular dichroism (CD) spectra and thermal melting profiles of guinea pigliver DNA reassociated with histones and/or nonhistone proteins from the cerebral of liver chromatin are described. 2. In the DNA-histone complex, positive ellipiticity in the CD spectrum at 260-300 nm is progressively lod by a red-shift of the crossover point at around 260 nm. DNA in this complex is thermally stabilised to a considerable extent, but not to such a full extent as is shown with DNA in native chromatin. 3. DNA-nonhistone complex in 0.14 M NaCl is, in contrast to DNA-histone complex, not precipitable by centrifugation at 20 000 X g. DNA in this complex shows only a slight reduction in ellipticity at 260-300 nm, and a very weak thermal stabilisation. 4. Characteristics in the CD spectrum of the native chromatin are most satisfactorily reproduced in the DNA-histone-nonhistone complex. These include a large decrease in ellipticity at 260-300 nm, a red-shift of the crossover point at around 260 nm, and a slight negative band at around 305 nm. Also, DNA in this complex is thermally stabilised to the extent comparable with DNA in the native chromatin. 5. Addition of nonhistone proteins to the preformed DNA-histone complex in 3 M urea ...Continue Reading

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Citations

Jul 1, 1977·Nucleic Acids Research·N DeferJ Brahms
May 1, 1978·Nucleic Acids Research·L L JagodzinskiJ S Sevall
Aug 4, 1981·Biochemistry·D P QuickJ A Duerre
Jan 1, 1978·Experimental Cell Research·G D Sinclair, K Brasch
Mar 1, 1977·Archives of Biochemistry and Biophysics·B Samal, I Bekhor

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