A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test

Memórias do Instituto Oswaldo Cruz
Bárbara C A AssisZ A Andrade

Abstract

A highly specific pattern of immunofluorescence was noted when sera from Capillaria hepatica-infected rats were tested against the homologous worms and eggs present either in paraffin or cryostat sections from mouse liver. The pattern was represented by a combined apple green fluorescence of the internal contents of worms and eggs, which persisted in serum-dilutions of 1:400 up to 1:1600. Unequivocal fluorescent pattern was observed from 15 days up to 3 months following inoculation of rats with embryonated C. hepatica eggs and such result was confirmed by the ELISA. After the 4th month of infection, the indirect immunofluorescence test turned negative, probably revealing the extinction of parasitism, however the ELISA was contradictory, disclosing high levels of antibodies in this period. The IIF was also negative when control normal rat sera and sera from rats administered by gavage with immature C. hepatica eggs (spurious infection), or for reactions made against Schistosoma mansoni eggs, although a weakly positive pattern occurred with Fasciola hepatica eggs. The indirect immunofluorescence test may be recommended for use with human sera to detect early C. hepatica infection in special clinical instances and in epidemiologic...Continue Reading

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Citations

Jul 1, 2011·Parasitology Research·Hans-Peter FuehrerHerbert Auer
Jan 19, 2008·Pathology·Luiz Cesar PeresAndrea de Faria Fernandes Belone
Jan 4, 2013·PLoS Neglected Tropical Diseases·Alessandra Queiroga GonçalvesPatricia Puccinelli Orlandi
Apr 2, 2015·International Journal for Parasitology. Parasites and Wildlife·Georg G DuscherAnja Joachim
Feb 5, 2015·Revista Do Instituto De Medicina Tropical De São Paulo·Elierson José Gomes da RochaLuís Marcelo Aranha Camargo

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