A controlled biochemical and immunohistochemical study of human synovial-type (group II) phospholipase A2 and inflammatory cells in macroscopically normal, degenerated, and herniated human lumbar disc tissues

Spine
M GrönbladR D Guyer

Abstract

Group II phospholipase A2 enzyme activity was studied biochemically and immunohistochemically in tissue samples from disc prolapses, degenerated discs, and macroscopically normal discs. In parallel, phospholipase A2 and inflammatory cells were studied by indirect immunocytochemistry. To compare phospholipase A2 activity in normal discs and abnormal discs by an identical assay for phospholipases A2, and to compare the occurrence of inflammatory cells with phospholipase A2 activity and immunoreactivity. It has been suggested that a high phospholipase A2 enzyme activity in herniated disc tissue could be significant in abnormal states such as sciatica and discogenic pain. No comparison between healthy disc tissue and samples of abnormal discs (degenerated or herniated) has been carried out. In particular, an identical assay for phospholipase A2 for such tissue samples, supported by immunohistochemical staining data, has never been applied in parallel to normal and abnormal disc tissue, and neither have such results been compared with the demonstration of inflammatory cells. Group II phospholipase A2 enzyme activity was determined, in parallel, using an identical assay for tissue samples from 11 macroscopically normal discs, 33 disc...Continue Reading

References

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Citations

Aug 19, 2004·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Itaru AraiK Olmarker
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·C F P TeixeiraY Cury
Jun 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M KawakamiT Tamaki
May 15, 1997·Spine·M I Jayson
Jul 9, 1999·Journal of Epidemiology and Community Health·H HemingwayM Marmot
May 27, 2005·Neurosurgical Focus·Michael D MartinDavid G Malone
Apr 23, 2005·Neurological Research·Ko-ichi IwatsukiRie Nakahira
May 28, 2010·Arthritis and Rheumatism·Kenneth M C CheungKeith D K Luk
Sep 25, 2004·Journal of Manipulative and Physiological Therapeutics·Paul DoughertyJ Donald Dishman
Mar 17, 2010·Best Practice & Research. Clinical Rheumatology·Jean-Pierre ValatBart Koes
May 13, 2017·Current Medical Research and Opinion·Cheng-Di ChiuChia-Hung Kao
Jun 4, 2010·European Journal of Anaesthesiology·Oonagh T HickeyGeorge D Shorten
Apr 20, 2004·The Journal of the American Academy of Orthopaedic Surgeons·Ashok Biyani, Gunnar B J Andersson
Sep 3, 1998·Seminars in Arthritis and Rheumatism·P GoupilleA J Freemont

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