A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
H TottyP Deol

Abstract

The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALERT® FN Plus (FN Plus), BacT/ALERT® Standard Aerobic (SA), and BacT/ALERT® Standard Anaerobic (SN) blood culture bottles (bioMérieux Inc., Durham, NC). A seeded limit of detection (LoD) study was performed for each bottle type in both systems. The LoD studies demonstrated that both systems were capable of detecting organisms at nearly identical levels [<10 colony-forming units (CFU) per bottle], with no significant difference. Following LoD determination, a seeded study was performed to compare the time to detection (TTD) between the systems using a panel of clinically relevant microorganisms inoculated at or near the LoD with 0, 4, or 10 mL of healthy human blood. VIRTUO™ exhibited a faster TTD by an average of 3.5 h, as well as demonstrated a significantly improved detection rate of 99.9% compared to 98.8% with 3D (p-value <0.05).

References

Jan 10, 2002·Infectious Disease Clinics of North America·R R Magadia, M P Weinstein
Mar 19, 2011·Journal of Medical Microbiology·Gabriella TerhesElisabeth Nagy
Jun 18, 2011·Journal of Medical Microbiology·Carla RosaCidália Pina-Vaz

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Citations

Jun 1, 2018·Annals of Saudi Medicine·Ali Mohammed SomilyA Khalifa Binkhamis
Aug 9, 2020·Platelets·Joels Wilson-NieuwenhuisNina Dempsey-Hibbert
Oct 10, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Mary AdamikParampal Deol

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