A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium.

Microbiology
Alessandra ArgnaniPeter H Pouwels

Abstract

A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4 degrees C. Transformation of Bifidobacterium could be achieved with a plasmid vector originating from Bifidobacterium and with plasmid vectors from Corynebacterium, but not with vectors carrying replicons from Lactococcus or Lactobacillus.

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Citations

Feb 21, 2006·Biotechnology Letters·Seong Lyul RhimGeun Eog Ji
Aug 19, 2007·Biotechnology Letters·Young-Seo ParkSung-Sik Yoon
Jun 9, 2005·FEMS Microbiology Reviews·Adrianne KlijnFabrizio Arigoni
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Oct 30, 2007·Applied and Environmental Microbiology·Michelle CroninDouwe van Sinderen
Feb 7, 2001·Applied and Environmental Microbiology·J P JoreP H Pouwels
Jan 5, 2006·Applied and Environmental Microbiology·Ju-Hoon Lee, Daniel J O'Sullivan
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Ju-Hoon Lee, Daniel J O'Sullivan
Mar 7, 2006·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Minoru Fujimori
Feb 6, 2014·International Journal of Food Microbiology·José M LandeteJuan L Arqués
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Dec 1, 2012·Microbiology·Wilfredo Dominguez, Daniel J O'Sullivan
Dec 29, 2012·Beneficial Microbes·S GuglielmettiP Álvarez-Martín
May 30, 2012·FEMS Microbiology Letters·Fausta SerafiniMarco Ventura
Jan 30, 2020·Biotechnology Journal·Sung Won ChoSang Woo Seo
Apr 10, 2021·Frontiers in Microbiology·Emily C HoedtDouwe van Sinderen

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