A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Molecular BioSystems
Ying HuangWenshe Liu

Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

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Citations

Feb 11, 2012·Analytical and Bioanalytical Chemistry·Hui-wang Ai
Mar 19, 2014·Biochimica Et Biophysica Acta·Wei WanWenshe R Liu
Sep 20, 2011·Nature Chemical Biology·David B F JohnsonLei Wang
Jan 13, 2012·Current Opinion in Biotechnology·Michael Georg Hoesl, Nediljko Budisa
Oct 26, 2012·Journal of Nucleic Acids·Takashi Kawakami, Hiroshi Murakami
Jun 19, 2013·Current Opinion in Chemical Biology·Dina GrohmannPhilip Tinnefeld
Jun 20, 2015·Trends in Biotechnology·Yuvaraj RavikumarHyungdon Yun
Apr 19, 2016·Annual Review of Biochemistry·Poonam BhedaHening Lin
Sep 3, 2014·Chembiochem : a European Journal of Chemical Biology·Tatsuo YanagisawaShigeyuki Yokoyama
Jul 22, 2014·Chembiochem : a European Journal of Chemical Biology·Yu ZengWenshe R Liu
Dec 24, 2015·Organic & Biomolecular Chemistry·Conor M HaneyE James Petersson
Nov 10, 2017·Antioxidants & Redox Signaling·David E WrightPatrick O'Donoghue
Aug 25, 2017·RNA Biology·Jeffery M TharpWenshe R Liu
Aug 17, 2010·Molecular BioSystems·Yane-Shih WangWenshe R Liu
Sep 25, 2014·The Journal of Biological Chemistry·Yun WangPhilip A Cole
Feb 3, 2015·Current Protocols in Protein Science·Ling Wu, Bin Xu
Mar 16, 2011·Angewandte Chemie·Michael G Hoesl, Nediljko Budisa
Jul 30, 2014·Chembiochem : a European Journal of Chemical Biology·Tatsuo YanagisawaShigeyuki Yokoyama
Jul 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hao ChenChenguang Fan
Feb 3, 2012·Angewandte Chemie·Karin V LoschaGottfried Otting
Mar 21, 2013·Angewandte Chemie·Moritz J Schmidt, Daniel Summerer
Jul 15, 2016·Cold Spring Harbor Perspectives in Biology·Han Xiao, Peter G Schultz
Jun 6, 2012·ACS Chemical Biology·David B F JohnsonLei Wang
Aug 25, 2018·ACS Synthetic Biology·Jessica T StieglitzJames A Van Deventer
May 7, 2015·ACS Chemical Biology·Byeong Sung LeeTae Hyeon Yoo

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