A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Molecular BioSystems
Ying HuangWenshe Liu


By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.


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Jun 27, 2007·Nature Biotechnology·Kaihang WangJason W Chin
Nov 7, 2007·Nature Structural & Molecular Biology·Sabbi Lall
Feb 19, 2008·Nature Chemical Biology·Heinz NeumannJason W Chin
Aug 30, 2008·Molecular Cell·Xiang-Jiao Yang, Edward Seto
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