A convenient method for preparation of biologically active recombinant CHH of the kuruma prawn, Marsupenaeus japonicus, using the bacterial expression system

Peptides
Chiaki NagaiHiromichi Nagasawa

Abstract

Crustacean hyperglycemic hormone (CHH) not only plays an important role in the modulation of hemolymph glucose level but also functions in other biological events including molting, reproduction and stress response. Of the six CHHs characterized in Marsupenaeus japonicus, an expression system for recombinant Pej-SGP-VII (rPej-SGP-VII-amide) has not yet been established. Here, we established a procedure using a Nus-tag for solubilization, thereby soluble and biologically active rPej-SGP-VII-amide could successfully be obtained by a simpler procedure than previous ones used for producing other recombinant Pej-SGPs (Pej-SGP-I, III and IV). It was found that rPej-SGP-VII-amide thus obtained had the correct arrangement of intramolecular disulfide bonds and helix-rich secondary structure. The established expression system for rPej-SGP-VII-amide may be applicable for the preparation of other recombinant CHHs.

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Citations

Dec 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hirotaka InoueHiromichi Nagasawa
Dec 15, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Natalia C PrymaczokDaniel A Medesani
Dec 2, 2015·Bioscience, Biotechnology, and Biochemistry·Hidekazu Katayama
Oct 28, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Vrinda SBright Singh I S
Oct 6, 2009·Archives of Insect Biochemistry and Physiology·Hideaki AsazumaHiromichi Nagasawa

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