PMID: 8471023Feb 1, 1993Paper

A correction for allele frequency estimates derived from isofemale lines

Biochemical Genetics
A D Long

Abstract

Isofemale lines are commonly used in Drosophila and other genera for the purpose of assaying genetic variation. Isofemale lines can be kept in the laboratory for many generations before genetic work is carried out, and permit the confirmation of newly discovered alleles. A problem not realized by many workers is that the commonly used estimate of allele frequency from these lines is biased. This estimation bias occurs at all times after the first laboratory generation, regardless of whether single individuals or pooled samples are used in each well of an electrophoretic gel. This bias can potentially affect the estimation of population genetic parameters, and in the case of rare allele analysis it can cause gross overestimates of gene flow. This paper provides a correction for allele frequency estimates derived from isofemale lines for any time after the lines are established in the laboratory. When pooled samples are used, this estimator performs better than the standard estimator at all times after the first generation. The estimator is also insensitive to multiple inseminations. After the lines have drifted one Ne generations, multiple inseminations actually make the new estimator perform better than it does in singly insemi...Continue Reading

References

Mar 1, 1982·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R C GriffithsJ A McKenzie
Jan 1, 1985·Evolution; International Journal of Organic Evolution·Montgomery Slatkin

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Citations

Aug 11, 2000·Evolution; International Journal of Organic Evolution·M A NoorK R Smith

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