A cost-effective and rapid quantitative test of polymyxin B sulphate with a simple and portable pressure meter readout.

Analytical Methods : Advancing Methods and Applications
Xinyue RaoZhongde Liu

Abstract

Any signals, if their intensities have a simple functional relationship with analyte concentration, can be applied for analytical purposes. Among them, pressure measurement as a signalling technique has been extensively utilized to develop portable and quantitative bioanalysis, with the advantages of highly sensitive detection of a variety of biomedical targets. In this contribution, it was found that polymyxin B sulphate (PMB) could significantly inhibit the catalytic ability of platinum nanoparticles (PtNPs) prepared in different protocols in the H2O2 breakdown reaction. By employing sodium citrate as the reducing agent to prepare the platinum nanoparticles (C-PtNPs) as an example and monitoring the pressure signal changes of the C-PtNP catalyzed H2O2 breakdown against the reaction time, a sensitive, cost-effective, rapid and reliable analysis method for PMB is established on the basis of the pressure signal readout. The proposed method has a detection limit of 28.6 nM and can selectively detect PMB in both POLY-MxB powder injection and human urine samples, demonstrating its potential in bioanalysis, which would be significant to address biological, clinical and medicinal requirements.

References

Jan 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·E AdamsJ Hoogmartens
Mar 18, 2000·Journal of Chromatography. a·J A OrwaJ Hoogmartens
Feb 28, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cindy GovaertsJos Hoogmartens
Nov 23, 2006·Journal of Pharmaceutical and Biomedical Analysis·Piyaporn SrisomSunanta Wangkarn
Oct 6, 2007·Diagnostic Microbiology and Infectious Disease·Andrea L H KwaVincent H Tam
Aug 22, 2012·Antimicrobial Agents and Chemotherapy·Kamilia AbdelraoufVincent H Tam
Jan 10, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·Ling KongFengling Tian
May 24, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ana M SandriAlexandre P Zavascki
Mar 28, 2018·ACS Applied Materials & Interfaces·Jiuxing LiChaoyong Yang
Apr 7, 2018·ACS Applied Materials & Interfaces·Lu ShiYan Jin

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Methods Mentioned

BETA
electrophoresis
biosensing
dynamic light scattering
transmission electron microscopy

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