A cost-effective cell- and matrix-based minimally invasive single-stage chondroregenerative technique developed with validated vertical translation methodology

Annals of the Royal College of Surgeons of England
Asode Ananthram ShettyH J Jang

Abstract

Introduction The morbidity and significant health economic impact associated with the chondral lesion has led to a large number of strategies for therapeutic neochondrogenesis. The challenge has been to develop techniques that are cost effective single-stage procedures with minimal surgical trauma that have undergone rigorous preclinical scrutiny and robust reproducible assessment of effectiveness. A biological repair requires the generation of a cellular and matrix composite with appropriate signalling for chondrogenic differentiation. Methods and results A technique was developed that allowed chondrogenic primary (uncultured) cells from bone marrow aspirate concentrate, combined with a composite hydrophilic and fibrillar matrix to be applied arthroscopically to a site of a chondral lesion. The construct was tested in vitro and in animal experiments before clinical trials. Clinical trials involved 60 patients in a prospective study. Symptomatic International Cartilage Repair Society grade 3 and 4a lesions were mapped and treated. Pre- and postoperative clinical assessments showed statistically significant improved outcomes; Lysholm Knee Scoring Scale (mean 52.8 to > 76.4; P < 0.05) International Knee Documentation Committee (m...Continue Reading

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Citations

Dec 29, 2020·Stem Cells and Cloning : Advances and Applications·Noor Buzaboon, Sfoug Alshammary
Aug 24, 2021·Emerging Topics in Life Sciences·Charlotte H HulmeSally Roberts

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
fluorescence-activated cell sorting
flow cytometry
bone marrow aspiration

Software Mentioned

MOCART

Related Concepts

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