A coumarin-based TICT fluorescent probe for real-time fluorescence lifetime imaging of mitochondrial viscosity and systemic inflammation in vivo.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Yun LiangWeiying Lin


Systemic inflammation, linked with abnormal mitochondrial viscosity, is reported to be associated with cerebro-cardiovascular disease and Alzheimer's disease. Therefore, it is of great significance to detect the mitochondrial viscosity to indicate the inflammatory signal in vivo. Considering the strategies of fluorescent molecular rotors (FMRs) and fluorescence lifetime imaging microscopy (FLIM), we have rationally designed a novel mitochondrial viscosity-specific fluorescent probe Mito-VCI, based on coumarin fluorophores with benzo[e]indolium as the rotor group. In a high viscosity solution system, the fluorescence lifetime of the probe Mito-VCI was prolonged due to the planarization and rigidity enhancement of the molecular rotor. Satisfactorily, the probe was only sensitive to viscosity, instead of non-viscosity factors such as pH and polarity. Furthermore, the probe sensitively targeted mitochondria in HeLa cells with a Pearson's correlation of 0.93, and specifically detected dynamics variation of mitochondrial viscosity with FLIM imaging in HeLa cells induced by LPS. Notably, significant fluorescence lifetime changes of Mito-VCI between normal and inflammatory tissues also occurred (for example, the fluorescence lifetime i...Continue Reading


Jun 16, 2005·Current Opinion in Cardiology·Patrick M Moriarty, Cheryl A Gibson
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
May 30, 2013·Journal of the American Chemical Society·Zhigang YangJong Seung Kim
Jun 13, 2013·Current Opinion in Microbiology·Suzanne M Cloonan, Augustine M K Choi
Apr 12, 2014·Chemical Society Reviews·Zhigang YangJong Seung Kim
May 6, 2015·Chemical Reviews·Hwan Myung Kim, Bong Rae Cho
Sep 24, 2016·ACS Medicinal Chemistry Letters·Xiang-Guo LiAnne Roivainen
Nov 5, 2016·Brain, Behavior, and Immunity·Elliot ElwoodIan Galea
Apr 12, 2017·Developmental Cell·Allyson L Anding, Eric H Baehrecke
Dec 15, 2017·Journal of the Royal Society, Interface·B Rita AlevriadouMuniswamy Madesh
Jul 15, 2018·Trends in Molecular Medicine·Nona M JiangWilliam A Petri
Jul 8, 2019·Brain, Behavior, and Immunity·Rachel Pei-Hsuan WangRaymond Chuen-Chung Chang
Jul 18, 2019·Chemical Reviews·Duxia CaoWeiying Lin
Jul 22, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Min PengWeiying Lin
May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rui GuoWeiying Lin
Sep 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenggong MaBaoxing Shen
Nov 17, 2020·Bioconjugate Chemistry·Cindy MaShana O Kelley
Apr 21, 2021·Journal of Agricultural and Food Chemistry·Xueyan WuGuang Yang

❮ Previous
Next ❯

Methods Mentioned

silica gel column chromatography
fluorescence imaging

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Alzheimer's Disease: Neuroimaging

Neuroimaging can help identify pathological hallmarks of Alzheimer's disease (AD). Here is the latest research on neuroimaging modalities, including magnetic resonance imaging and positron emission tomography, in AD.

Related Papers

Journal of Visualized Experiments : JoVE
K SuhlingGokhan Yahioglu
Analytical Methods : Advancing Methods and Applications
Lin YuanZhengjie Liu
Advances in Experimental Medicine and Biology
Marina V ShirmanovaMarina K Kuimova
© 2021 Meta ULC. All rights reserved