PMID: 8962073Dec 10, 1996Paper

A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis

Proceedings of the National Academy of Sciences of the United States of America
F DongP H von Hippel

Abstract

We have developed a coupled helicase-polymerase DNA unwinding assay and have used it to monitor the rate of double-stranded DNA unwinding catalyzed by the phage T4 DNA replication helicase (gp41). This procedure can be used to follow helicase activity in subpopulations in systems in which the unwinding-synthesis reaction is not synchronized on all the substrate-template molecules. We show that T4 replication helicase (gp41) and polymerase (gp43) can be assembled onto a loading site located near the end of a long double-stranded DNA template in the presence of a macro-molecular crowding agent, and that this coupled "two-protein" system can carry out ATP-dependent strand displacement DNA synthesis at physiological rates (400 to 500 bp per sec) and with high processivity in the absence of other T4 DNA replication proteins. These results suggest that a direct helicase-polymerase interaction may be central to fast and processive double-stranded DNA replication, and lead us to reconsider the roles of the other replication proteins in processivity control.

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Citations

Jul 17, 1998·Current Opinion in Chemical Biology·D J SextonS J Benkovic
Aug 5, 2010·Quarterly Reviews of Biophysics·Jaya G YodhTaekjip Ha
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ajit K SatapathyCharles C Richardson
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Davis JosePeter H von Hippel
Jul 27, 2007·Molecular Biology of the Cell·Ben HodgsonKarim Labib
Jul 17, 2008·Genes & Development·Shawn J SzyjkaOscar M Aparicio
Aug 3, 2010·Journal of Bacteriology·Stephan KolkenbrockSusanne Fetzner
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Mar 21, 2009·Annual Review of Biochemistry·Samir M Hamdan, Charles C Richardson
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C E JonesN G Nossal
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Timothée LionnetVincent Croquette
Aug 26, 2011·Current Opinion in Chemical Biology·Smita S PatelDivya Nandakumar
Feb 26, 1998·Cell·T A Baker, S P Bell
Apr 8, 2000·Trends in Biochemical Sciences·K N Kreuzer
Jun 24, 2015·Viruses·Erin NobleStephen J Benkovic
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Peter H von Hippel, Emmanuelle Delagoutte
Sep 23, 2003·The Journal of Biological Chemistry·Jingsong YangStephen J Benkovic
May 4, 2006·The Journal of Biological Chemistry·Smita S Patel, Ilker Donmez
Nov 18, 2018·Nature Communications·Silvia Emma RossiMichele Giannattasio
Apr 18, 2000·The Journal of General Virology·C PaoliniP Gallinari
Dec 20, 2020·Biophysical Journal·Keith J MickolajczykTarun M Kapoor

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