PMID: 9636133Jun 24, 1998Paper

A coupled proton-transfer and twisting-motion fluorescence probe for lipid bilayers

Proceedings of the National Academy of Sciences of the United States of America
C R Mateo, A Douhal

Abstract

A new and sensitive molecular probe, 2-(2'-hydroxyphenyl)imidazo[1, 2-a]pyridine (HPIP), for monitoring structural changes in lipid bilayers is presented. Migration of HPIP from water into vesicles involves rupture of hydrogen (H) bonds with water and formation of an internal H bond once the probe is inside the vesicle. These structural changes of the dye allow the occurrence of a photoinduced intramolecular proton-transfer reaction and a subsequent twisting/rotational process upon electronic excitation of the probe. The resulting large Stokes-shifted fluorescence band depends on the twisting motion of the zwitterionic phototautomer and is characterized in vesicles of dimyristoyl-phosphatidylcholine and in dipalmitoyl-phosphatidylcholine at the temperature range of interest and in the presence of cholesterol. Because the fluorescence of aqueous HPIP does not interfere in the emission of the probe within the vesicles, HPIP proton-transfer/twisting motion fluorescence directly allows us to monitor and quantify structural changes within bilayers. The static and dynamic fluorescence parameters are sensitive enough to such changes to suggest this photostable dye as a potential molecular probe of the physical properties of lipid bila...Continue Reading

References

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Citations

Sep 25, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Cristina MartínAbderrazzak Douhal
Jan 30, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·Alexander P Demchenko
Nov 2, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Dmytro A YushchenkoVasyl G Pivovarenko
Jan 24, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bijan Kumar Paul, Nikhil Guchhait
Nov 26, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Monalisa Mohapatra, Ashok Kumar Mishra
Oct 10, 2008·The Journal of Physical Chemistry. B·Laura TormoAbderrazzak Douhal
Apr 15, 2004·Chemical Reviews·Abderrazzak Douhal
Jan 11, 2011·The Journal of Physical Chemistry. B·Dipanwita De, Anindya Datta
Jul 21, 2006·The Journal of Physical Chemistry. B·Christopher J Wohl, Darius Kuciauskas
Oct 28, 2021·Organic & Biomolecular Chemistry·Javeed Ahmad TaliRavi Shankar

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