A COVID-19 patient with multiple negative results for PCR assays outside Wuhan, China: a case report

BMC Infectious Diseases
Li-Da ChenLi Lin

Abstract

The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a public health emergency of major international concern. Real-time RT-PCR assays are recommended for diagnosis of COVID-19. Here we report a rare case of COVID-19 with multiple negative results for PCR assays outside Wuhan, China. A 32-year old male was admitted to our hospital because of 6 days of unexplained fever on January 29, 2020. He had come from Wuhan city 10 days before admission. Five days before admission, no abnormality was noted in laboratory test, chest radiography, and nasopharyngeal swab test for the SARS-CoV-2 nucleic acid. The patient was treated with ibuprofen for alleviating fever. On admission, chest computed tomography showed multiple ground-glass opacities in right lower lung field. COVID-19 was suspected. Three times of nasopharyngeal swab specimens were collected after admission. However, none of the specimens were positive. The patient was confirmed with COVID-19 after fifth SARS-CoV-2 nucleic acid test. He was treated with lopinavir/ritonavir, recombinant human interferon alfa-2b inhalation, methylprednisolone. After 18 days of treatment, he was discharged with improve...Continue Reading

References

Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 28, 2020·The Lancet Infectious Diseases·Yang PanQuanyi Wang
Feb 29, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Xi XuJinxin Liu
Feb 29, 2020·The New England Journal of Medicine·Wei-Jie GuanUNKNOWN China Medical Treatment Expert Group for Covid-19

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Citations

Aug 8, 2020·Journal of Medical Virology·Arantxa ValdiviaDavid Navarro
Sep 9, 2020·Journal of Medical Virology·Mohamed ArafkasVeria Khosrawipour
Aug 10, 2020·ANZ Journal of Surgery·Joshua G KovoorGuy J Maddern
Aug 21, 2020·Journal of Clinical Microbiology·Matthew J Binnicker
Jun 1, 2021·The Lancet Infectious Diseases·Fernando Kemta LekpaUNKNOWN University of Dschang Taskforce for the Elimination of COVID-19 (UNITED#COVID-19)
May 11, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Eimear M KittKathleen Chiotos
Sep 10, 2021·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Vivek B KuteHari Shankar Meshram
Oct 1, 2021·Cell and Tissue Banking·Sheila F O'BrienSteven J Drews

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Methods Mentioned

BETA
PCR
nucleic

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