A CRE/AP-1-like motif is essential for induced syncytin-2 expression and fusion in human trophoblast-like model.

PloS One
Chirine ToufailyBenoit Barbeau

Abstract

Syncytin-2 is encoded by the envelope gene of Endogenous Retrovirus-FRD (ERVFRD-1) and plays a critical role in fusion of placental trophoblasts leading to the formation of the multinucleated syncytiotrophoblast. Its expression is consequently regulated in a strict manner. In the present study, we have identified a forskolin-responsive region located between positions -300 to -150 in the Syncytin-2 promoter region. This 150 bp region in the context of a minimal promoter mediated an 80-fold induction of promoter activity following forskolin stimulation. EMSA analyses with competition experiments with nuclear extracts from forskolin-stimulated BeWo cells demonstrated that the -211 to -177 region specifically bound two forskolin-induced complexes, one of them containing a CRE/AP-1-like motif. Site-directed mutagenesis of the CRE/AP-1 binding site in the context of the Syncytin-2 promoter or a heterologous promoter showed that this motif was mostly essential for forskolin-induced promoter activity. Transfection experiments with dominant negative mutants and constitutively activated CREB expression vectors in addition to Chromatin Immunoprecipitation suggested that a CREB family member, CREB2 was binding and acting through the CRE/A...Continue Reading

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Citations

Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Robin A Weiss
Aug 4, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Bikem Soygur, Leyla Sati
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Jun 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hua ZhuPeter C K Leung

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Methods Mentioned

BETA
flow cytometry
PCR
electrophoretic mobility shift assay
Immunoprecipitation
transfection
transfections
confocal microscopy
ChIP
acetylation
histone acetylation

Software Mentioned

Quantity One
GraphPad prism

Related Concepts

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