A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus

Scientific Reports
Xun LiangGang Cao

Abstract

Virus evolves rapidly to escape vaccine-induced immunity, posing a desperate demand for efficient vaccine development biotechnologies. Here we present an express vaccine development strategy based on CRISPR/Cas9 and Cre/Lox system against re-emerging Pseudorabies virus, which caused the recent devastating swine pseudorabies outbreak in China. By CRISPR/Cas9 system, the virulent genes of the newly isolated strain were simultaneously substituted by marker genes, which were subsequently excised using Cre/Lox system for vaccine safety concern. Notably, single cell FACS technology was applied to further promote virus purification efficiency. The combination of these state-of-art technologies greatly accelerated vaccine development. Finally, vaccination and challenge experiments proved this vaccine candidate's protective efficacy in pigs and the promise to control current pseudorabies outbreak. This is, to our knowledge, the first successful vaccine development based on gene edit technologies, demonstrating these technologies leap from laboratory to industry. It may pave the way for future express antiviral vaccine development.

References

Jan 1, 1987·Annual Review of Microbiology·D A Steinhauer, J J Holland
Nov 1, 1996·Veterinary Immunology and Immunopathology·T C Mettenleiter
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·G A Smith, L W Enquist
Oct 16, 2002·Trends in Microbiology·Mark E J Woolhouse
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Lisa E PomeranzChristoph J Hengartner
Sep 1, 2006·Cell Biochemistry and Function·Agostino PuglieseDonato Torre
Mar 13, 2012·Biotechnology and Bioengineering·Jessica O Josefsberg, Barry Buckland
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Jan 31, 2013·Nature Biotechnology·Woong Y HwangJ Keith Joung
Mar 6, 2013·Nucleic Acids Research·James E DiCarloGeorge M Church
May 28, 2013·Genetics·Scott J GratzKate M O'Connor-Giles
Jul 4, 2013·Journal of Veterinary Science·Rui WuXuke Zhang
Aug 10, 2013·Nature Biotechnology·Dali LiMingyao Liu
Aug 21, 2013·Cell Research·Zhengyan FengJian-Kang Zhu
Jan 1, 2014·Emerging Infectious Diseases·Xiuling YuKegong Tian
Apr 18, 2014·The New England Journal of Medicine·Sylvain BaizeStephan Günther
Jul 6, 2014·Current Protocols in Neuroscience·J Patrick Card, Lynn W Enquist
Oct 3, 2014·Nature·Mitchell R O'ConnellJennifer A Doudna
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aryn A PriceDavid S Weiss
May 29, 2015·PLoS Pathogens·Austin G Meyer, Claus O Wilke
Jun 4, 2015·Scientific Reports·Vyas RamananSangeeta N Bhatia
Jun 25, 2015·Microbiology Spectrum·Gregory D Van Duyne

❮ Previous
Next ❯

Citations

May 11, 2016·Chemical Reviews·Gretchen MeinkeFrank Buchholz
Sep 14, 2016·Virus Research·Yan-Dong TangXue-Hui Cai
Nov 29, 2016·Veterinary Microbiology·C M FreulingT C Mettenleiter
Mar 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan-Dong TangXue-Hui Cai
Mar 30, 2018·Journal of Biomedical Science·Sehrish KhanAftab Ahmad
Apr 5, 2016·Oral Diseases·A Peterson
Nov 28, 2018·Virus Genes·Chao YeGuangzhi Tong
Jul 17, 2020·Frontiers in Immunology·Richard B KennedyGregory A Poland
Oct 7, 2020·Animals : an Open Access Journal From MDPI·Fengsun WuWengang Li
Dec 4, 2020·Nucleic Acids Research·Amer EliasMikhail Kolot
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Julianne VilelaMuhammad Munir
Jun 20, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jinfu ZhangShengsong Xie

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FACS
transfection
single cell
fluorescence-activated cell sorting

Software Mentioned

BioEdit
GraphPad Prism

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Virology & CRISPR

This feed focuses on the virology of CRISPR and its use in developing CRISPR-Cas systems. Discover the latest research here.

Related Papers

Emerging Infectious Diseases
Xiuling YuKegong Tian
Veterinary Medicine, Small Animal Clinician : VM, SAC
R D Glock, R C Dillman
Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Vétérinaire
M D McFarland, H T Hill
© 2022 Meta ULC. All rights reserved