A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Satoshi KamijoHaruhiko Bito

Abstract

Despite many association studies linking gene polymorphisms and mutations of L-type voltage-gated Ca2+ channels (VGCCs) in neurodevelopmental disorders such as autism and schizophrenia, the roles of specific L-type VGCC during brain development remain unclear. Calcium signaling has been shown to be essential for neurodevelopmental processes such as sculpting of neurites, functional wiring, and fine tuning of growing networks. To investigate this relationship, we performed submembraneous calcium imaging using a membrane-tethered genetically encoded calcium indicator (GECI) Lck-G-CaMP7. We successfully recorded spontaneous regenerative calcium transients (SRCaTs) in developing mouse excitatory cortical neurons prepared from both sexes before synapse formation. SRCaTs originated locally in immature neurites independently of somatic calcium rises and were significantly more elevated in the axons than in dendrites. SRCaTs were not blocked by tetrodoxin, a Na+ channel blocker, but were strongly inhibited by hyperpolarization, suggesting a voltage-dependent source. Pharmacological and genetic manipulations revealed the critical importance of the Cav1.2 (CACNA1C) pore-forming subunit of L-type VGCCs, which were indeed expressed in imma...Continue Reading

Citations

Feb 13, 2019·Journal of Biochemistry·Shin-Ichiro HoriganeSayaka Takemoto-Kimura
Jul 13, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jihae OhBong-Kiun Kaang
Mar 17, 2020·Cellular and Molecular Neurobiology·Mitra Sadat Tabatabaee, Frederic Menard
Mar 20, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Benjamin A ElserHanna E Stevens
Aug 14, 2019·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Michihiro Igarashi
Nov 29, 2020·Cellular and Molecular Life Sciences : CMLS·Valeria KalteziotiPanagiotis K Politis
Jan 13, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Mitra Sadat TabatabaeeFrederic Menard
Feb 9, 2020·Neuroscience and Biobehavioral Reviews·Stefan KloiberAndre F Carvalho
Jan 5, 2022·Cell Regeneration·Xiaoxiang LuYangfei Xiang

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