A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells

FEBS Letters
K HiranoH Kanaide

Abstract

The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.

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Citations

Jan 26, 2011·Drug Discovery Today·Roy van der MeelAnnette T Byrne
Oct 7, 2004·Biochemical and Biophysical Research Communications·Miho KogaHideo Kanaide
Feb 18, 2006·Biochemical and Biophysical Research Communications·Fumi MomotaHideo Kanaide
Feb 24, 2015·Molecular and Cellular Biochemistry·Kana KuwaharaYasuo Shinohara
Apr 30, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Tatsuya YufuHideo Kanaide
Feb 24, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Katsuya HiranoHideo Kanaide
May 15, 2007·The Journal of Pharmacology and Experimental Therapeutics·Katsuya HiranoHideo Kanaide

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