A critical role of SRC-suppressed C kinase substrate in rat astrocytes after chronic constriction injury.

Neuromolecular Medicine
Yinyin XiaChun Cheng

Abstract

Src-suppressed C kinase substrate (SSeCKS), is an in vivo and in vitro protein kinase C substrate that may have a role in both mitogenic regulation and cytoskeletal arrangement. In this study, we mainly investigated the mRNA and protein expression and cellular localization of SSeCKS during chronic constriction injury (CCI). Reverse transcriptase-mediated PCR and western blot analysis revealed that SSeCKS was present in normal whole spinal cord. It gradually increased, and reached a peak at 2 weeks for its mRNA level and 7 days for its protein level after CCI. The protein expression of SSeCKS was further analyzed by immunohistochemistry. The positively stained areas for SSeCKS changed with the similar pattern to that of protein expression detected by immunoblotting analysis. Double immunofluorescence staining showed SSeCKS immunoreactivity was mostly co-localized with neurons, partly with activated astrocytes and rarely with microglia in the superficial laminar of spinal dorsal horn. In cell culture, the expression of pro-inflammation cytokines, p-ERK, and SSeCKS was increased in the spinal astrocytes after stimulated by damaged sensory neurons. However, SSeCKS gene silencing by siRNA inhibited the up-regulation of p-ERK and the...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P LiebermanM L Shin
Sep 1, 1980·The Journal of Pharmacy and Pharmacology·D C Faulkner, J W Growcott
Sep 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J A DeLeoA Malhotra
Jun 10, 2000·Science·C J Woolf, M W Salter
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S J LeeE N Benveniste
Nov 8, 2001·European Journal of Pharmacology·M Zimmermann
Jan 30, 2002·Brain Research·Sandra M SiegelPatrick A Carr
Oct 31, 2002·Nature Neuroscience·Joachim Scholz, Clifford J Woolf
Mar 19, 2003·British Journal of Pharmacology·A CiruelaK Lee
Apr 23, 2003·The American Journal of Pathology·Bing ZhuMax S Cynader
May 8, 2003·The Journal of Pharmacology and Experimental Therapeutics·Vasudeva RaghavendraJoyce A DeLeo
Dec 5, 2003·Nature Reviews. Drug Discovery·Linda R Watkins, Steven F Maier
Jan 26, 2005·Trends in Neurosciences·Makoto TsudaMichael W Salter
Jul 5, 2005·Nature Reviews. Neuroscience·Fabien MarchandStephen B McMahon
Feb 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David I HughesAndrew J Todd
Mar 28, 2008·Journal of Neurochemistry·Koichi ObataKoichi Noguchi

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