A Cross-sectional Pilot Cohort Study Comparing Standard Urine Collection to the Peezy Midstream Device for Research Studies Involving Women

Female Pelvic Medicine & Reconstructive Surgery
Elizabeth SouthworthElizabeth R Mueller

Abstract

The expanded quantitative urine culture protocol was used to compare the microbial abundance and diversity of voided urines obtained using a standard urine collection or using the Peezy midstream device versus paired periurethral specimens. Sixty-two female participants were assigned to 1 of 3 cohorts. One cohort used a standard clean catch midstream urine protocol that included a castile soap wipe, the second cohort used a Peezy midstream collection device with castile soap wipe and the third used the Peezy device without a castile soap wipe. Each participant watched a video that detailed the collection method. Before using the castile soap wipe, a periurethral swab was obtained to measure periurethral microbial abundance. Demographics and pelvic floor symptoms were assessed by validated questionnaires. Microbes were detected using expanded quantitative urine culture. Diversity within each sample was analyzed using alpha diversity measures. Paired periurethral and urine samples for each woman were analyzed and compared for species abundance, richness, and diversity. Bacterial profiles of Peezy-collected urines differed significantly by multiple diversity indices and had significantly reduced colony-forming units compared to pa...Continue Reading

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Citations

Aug 31, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·T K PriceL Brubaker
Apr 23, 2020·MBio·Travis K PriceAlan J Wolfe
May 11, 2019·Nature Reviews. Urology·Andrea GarrettoCatherine Putonti
Mar 3, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L BrubakerA J Wolfe
Mar 31, 2021·Nature Reviews. Urology·Naveen KachrooAaron W Miller
Apr 15, 2021·International Urogynecology Journal·Baylie R HochstedlerLinda Brubaker
Apr 27, 2021·STAR Protocols·Kait F Al, Jeremy P Burton
Jul 21, 2021·MSystems·Linda BrubakerAlan J Wolfe
Sep 1, 2021·Journal of Medical Engineering & Technology·Christopher Forrester, Aziza Mahomed

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