A cross-taxa study using environmental DNA/RNA metabarcoding to measure biological impacts of offshore oil and gas drilling and production operations

Marine Pollution Bulletin
Olivier LarocheX Pochon

Abstract

Standardized ecosystem-based monitoring surveys are critical for providing information on marine ecosystem health. Environmental DNA/RNA (eDNA/eRNA) metabarcoding may facilitate such surveys by quickly and effectively characterizing multi-trophic levels. In this study, we assessed the suitability of eDNA/eRNA metabarcoding to evaluate changes in benthic assemblages of bacteria, Foraminifera and other eukaryotes along transects at three offshore oil and gas (O&G) drilling and production sites, and compared these to morphologically characterized macro-faunal assemblages. Bacterial communities were the most responsive to O&G activities, followed by Foraminifera, and macro-fauna (the latter assessed by morphology). The molecular approach enabled detection of hydrocarbon degrading taxa such as the bacteria Alcanivorax and Microbulbifer at petroleum impacted stations. Most identified indicator taxa, notably among macro-fauna, were highly specific to site conditions. Based on our results we suggest that eDNA/eRNA metabarcoding can be used as a stand-alone method for biodiversity assessment or as a complement to morphology-based monitoring approaches.

Citations

Dec 26, 2018·Scientific Reports·Roberta PireddaAdriana Zingone
Sep 17, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Paula C FureySylvia Lee
Jun 28, 2019·Applied Microbiology and Biotechnology·Carmen Astudillo-GarcíaGavin Lear
Mar 25, 2021·Trends in Ecology & Evolution·Matthew C YatesMelania E Cristescu

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