A customizable method to characterize Arabidopsis thaliana transpiration under drought conditions

Plant Methods
Carlos de OllasAurelio Gómez-Cadenas

Abstract

Characterization of the dynamic response of plant transpiration to decreasing soil water content in a reproducible way is required for the correct phenotyping of traits related to water saving strategies. Nowadays, an increasing number of automated high throughput platforms are available, but their development requires a great economic investment and it is not always desirable/feasible to outsource these analyses. We propose a medium-throughput protocol to characterize transpiration responses to decreasing soil moisture in a quantitative and highly reproducible way with a minimum investment of resources. The quantitative characterization of plant responses to a decreasing soil water content using our phenotyping platform has showed high reproducibility between different experiments. The proposed irrigation strategy allowed us to harvest plants ranging from a well-watered condition to the loss-of-turgor point in a predictable and controlled way. Coupling this protocol with hormone profiling allows investigation of hormonal responses (metabolite accumulation as well as plant sensitivity) to water stress. As a proof-of-concept, we have characterized the dynamic responses of leaf transpiration to decreasing soil water contents in a...Continue Reading

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D Rock, J A Zeevaart
Oct 27, 2005·Journal of Agricultural and Food Chemistry·Abhilasha DurgbanshiAurelio Gómez-Cadenas
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Paul E VersluesJian-Kang Zhu
Mar 3, 2010·Annual Review of Plant Biology·Sean R CutlerSuzanne R Abrams
May 30, 2013·Frontiers in Plant Science·Agata Daszkowska-Golec, Iwona Szarejko
Mar 7, 2014·Journal of Experimental Botany·Vincent VadezHanna Anderberg
Sep 10, 2014·Applications in Plant Sciences·Hsien Ming Easlon, Arnold J Bloom
Feb 11, 2015·Annual Review of Plant Biology·Yoshiyuki MurataShintaro Munemasa
Jun 15, 2016·Plant Molecular Biology·Carlos de Ollas, Ian C Dodd
Aug 9, 2016·Journal of Experimental Botany·Sara I ZandalinasRon Mittler
Apr 19, 2017·Journal of Experimental Botany·Jaime PuértolasIan C Dodd
Sep 14, 2017·Plant Physiology·Aude Coupel-LedruThierry Simonneau
Jan 13, 2018·Journal of Experimental Botany·Abraham Blum, Roberto Tuberosa
Feb 13, 2018·Journal of Experimental Botany·Carlos De OllasIan C Dodd
Feb 26, 2019·Frontiers in Plant Science·Xinyun QuGuodong Wang
Mar 7, 2019·Frontiers in Plant Science·Jerry L Hatfield, Christian Dold
Oct 1, 2014·Functional Plant Biology : FPB·Abraham Blum

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Citations

Feb 3, 2021·Journal of Experimental Botany·Irene Garcia-MaquilonPedro L Rodriguez
Mar 17, 2021·Plant Cell Reports·Nguyen Nguyen ChuongNguyen Phuong Thao

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Methods Mentioned

BETA
gas

Software Mentioned

Easy Area
Masslynx
Easy Leaf area
Phenopsis
- GP
GP
Leaf
Sigmaplot

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