A Customized Self-Assembling Peptide Hydrogel-Wrapped Stem Cell Factor Targeting Pulp Regeneration Rich in Vascular-Like Structures

ACS Omega
Xiaodan MuXiumei Wang

Abstract

Pulp regeneration is to replace the inflamed/necrotic pulp tissue with regenerated pulp-like tissue to rejuvenate the teeth. Self-assembling peptide hydrogels RADA16-I (Ac-(RADA16-I)4-CONH2) can provide a three-dimensional environment for cells. The stem cell factor (SCF) plays a crucial role in homing stem cells. Combining these advantages, our study investigated the effects of SCF-RADA16-I on adhesion, proliferation, and migration of human dental pulp stem cells (DPSCs) and the angiogenesis of human umbilical vein endothelial cells (HUVECs). The β-sheet and grid structure were observed by circular dichroism (CD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Cytoskeleton staining, living cell staining, cell viability, cell migration, angiogenesis, and western blot assays were performed, and the results indicated that all the SCF groups were superior to the corresponding non-SCF groups in cell adhesion, proliferation, migration, and angiogenesis. RADA16-I provided a three-dimensional environment for DPSCs. Besides, the SCF promoted HUVECs to form more vascular-like structures and release more vascular endothelial growth factor A. In summary, the SCF-loaded RADA16-I scaffold improved adhesion, prolifera...Continue Reading

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Citations

Dec 16, 2020·Polymers·Marwa M S AbbassKarim M Fawzy El-Sayed
Jan 23, 2021·International Journal of Molecular Sciences·Oana BaruMîndra Badea
May 10, 2021·Advanced Drug Delivery Reviews·Christina Karavasili, Dimitrios G Fatouros
Jun 19, 2021·Soft Matter·Günnur Onak PulatOzan Karaman

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Methods Mentioned

BETA
AFM
Assay
protein assay

Software Mentioned

GraphPad Prism
Image J

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