A cut above the rest: targeted genome editing technologies in human pluripotent stem cells.

The Journal of Biological Chemistry
Mo LiJuan Carlos Izpisua Belmonte

Abstract

Human pluripotent stem cells (hPSCs) offer unprecedented opportunities to study cellular differentiation and model human diseases. The ability to precisely modify any genomic sequence holds the key to realizing the full potential of hPSCs. Thanks to the rapid development of novel genome editing technologies driven by the enormous interest in the hPSC field, genome editing in hPSCs has evolved from being a daunting task a few years ago to a routine procedure in most laboratories. Here, we provide an overview of the mainstream genome editing tools, including zinc finger nucleases, transcription activator-like effector nucleases, clustered regularly interspaced short palindromic repeat/CAS9 RNA-guided nucleases, and helper-dependent adenoviral vectors. We discuss the features and limitations of these technologies, as well as how these factors influence the utility of these tools in basic research and therapies.

References

Sep 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D W Melton
Feb 11, 2003·Nature Biotechnology·Thomas P Zwaka, James A Thomson
Oct 19, 2005·Gene Therapy·R AlbaM Chillon
Apr 28, 2006·Nature·Niels-Bjarne WoodsInder M Verma
May 1, 2008·Nature Methods·Cherie L RamirezJ Keith Joung
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Keiichiro SuzukiKohnosuke Mitani
Jan 2, 2009·Advances in Immunology·Katheryn MeekSusan P Lees-Miller
Jun 23, 2009·Stem Cells·Katherine M Ruby, Binhai Zheng
Aug 22, 2009·Biochemical and Biophysical Research Communications·Kaoru MitsuiKohnosuke Mitani
Nov 26, 2009·Science·Matthew J Moscou, Adam J Bogdanove
Mar 11, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lisa M PetekDaniel G Miller
Apr 22, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Iram F KhanDavid W Russell
Aug 19, 2010·Nature Reviews. Genetics·Fyodor D UrnovPhilip D Gregory
Mar 11, 2011·Clinical Pharmacology and Therapeutics·I Sancho-MartinezJ C Izpisua Belmonte
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sara E HowdenJames A Thomson
May 21, 2011·Cell Stem Cell·Guang-Hui LiuJuan Carlos Izpisua Belmonte
Aug 5, 2011·Nucleic Acids Research·Claudio MussolinoToni Cathomen
Aug 9, 2011·Nature Biotechnology·Richard GabrielChristof von Kalle
Aug 11, 2011·Genetics·Dana Carroll
Jan 10, 2012·Science·Amanda Nga-Sze MakBarry L Stoddard
Feb 18, 2012·Nature·Blake WiedenheftJennifer A Doudna
Mar 1, 2012·Nucleic Acids Research·Cherie L RamirezJ Keith Joung
Apr 10, 2012·Nature Biotechnology·Deepak ReyonJ Keith Joung
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jul 24, 2012·Current Opinion in Chemical Biology·Pablo Perez-PineraCharles A Gersbach
Aug 28, 2012·Molecular Cell·J Ross ChapmanSimon J Boulton
Sep 25, 2012·Blood·Chan-Jung Chang, Eric E Bouhassira
Sep 29, 2012·The Journal of Biological Chemistry·Julien ValtonPhilippe Duchateau
Oct 19, 2012·Nature·Guang-Hui LiuJuan Carlos Izpisua Belmonte
Nov 22, 2012·Nature Reviews. Molecular Cell Biology·J Keith Joung, Jeffry D Sander
Dec 1, 2012·Science·Frank Soldner, Rudolf Jaenisch
Dec 12, 2012·Genome Research·Mital S BhaktaDavid J Segal

❮ Previous
Next ❯

Citations

May 13, 2014·Blood Cancer Journal·D FocosiM Pistello
Aug 8, 2014·Nature Medicine·Stefanie DimmelerAlan Trounson
Jul 3, 2015·Expert Opinion on Biological Therapy·Dorota JeziorowskaJean-Sébastien Hulot
Dec 22, 2015·Biochemical and Biophysical Research Communications·Makoto HondaKazuhiro Aiba
Dec 9, 2015·Annual Review of Biomedical Engineering·Nenad BursacThomas A Rando
Sep 26, 2015·Annual Review of Genetics·Akitsu Hotta, Shinya Yamanaka
Jun 10, 2015·The American Journal of Pathology·Derek K Smith, Chun-Li Zhang
Dec 15, 2015·Frontiers in Veterinary Science·Dean H Betts, Ian C Tobias
Oct 16, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yuan Yan SinColin D Funk
Sep 30, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Koji TanabeMarius Wernig
Jul 26, 2015·Journal of Cell Communication and Signaling·Herman Yeger
Mar 21, 2015·Stem Cells and Development·Joshua G HunsbergerMahendra Rao
May 6, 2015·Surgical Oncology·Eric B Kmiec
Feb 26, 2015·Journal of Medical Genetics·Lu Xiao-JieJi Li-Juan
Jan 28, 2015·Developmental Biology·Matthew J Birket, Christine L Mummery
Jan 31, 2015·Cell Research·Lixia WangJuan Carlos Izpisua Belmonte
Feb 28, 2015·Protein & Cell·Liping DengGuang-Hui Liu
Dec 17, 2014·Frontiers in Pharmacology·Benjamin S JonesAntonio Di Stasi
Dec 8, 2014·Biochimica Et Biophysica Acta·Pranav Mathur, Jun Yang
May 14, 2016·Experimental Neurology·M Joana Osorio, Steven A Goldman
Jul 4, 2016·Journal of Huntington's Disease·Adelaide Tousley, Kimberly B Kegel-Gleason
Oct 19, 2016·Nature Reviews. Neuroscience·Prashanth RajarajanSchahram Akbarian
Jan 4, 2017·Nature Reviews. Genetics·Mo Li, Juan Carlos Izpisua Belmonte
Feb 28, 2015·Stem Cells Translational Medicine·Melissa K Carpenter, Mahendra S Rao
Jan 17, 2017·Current Urology Reports·Ximo García-DomínguezFrancisco Marco-Jimenez
Feb 9, 2017·Cells·Nicholas BrookhouserDavid A Brafman
Jan 1, 2014·Journal of Clinical Medicine·Dmitry A Ovchinnikov, Ernst J Wolvetang
Mar 30, 2018·Nature Cell Biology·Mo Li, Juan Carlos Izpisua Belmonte
Jan 8, 2016·Biomarker Insights·Benjamin S Freedman
Jun 19, 2015·Biomarker Insights·Jonas Schwan, Stuart G Campbell
Jul 2, 2014·Cell Research·Jinzhi DuanYu Zhang
Jun 27, 2018·The Journal of Biological Chemistry·Shuang WangYongchang Chen
Feb 4, 2016·Circulation Research·Mo Li, Juan Carlos Izpisua Belmonte

❮ Previous
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