PMID: 2503824Aug 1, 1989Paper

A cyanobacterial mutant requiring the expression of ribulose bisphosphate carboxylase from a photosynthetic anaerobe

Proceedings of the National Academy of Sciences of the United States of America
J PierceJ G Williams

Abstract

Ribulose bisphosphate carboxylase is essential for both photoautotrophic and photoheterotrophic growth of the cyanobacterium Synechocystis 6803. However, a mutant lacking cyanobacterial carboxylase could be obtained by replacing the natural carboxylase gene with the corresponding gene from Rhodospirillum rubrum, a photosynthetic anaerobe. This treatment produced an organism whose growth depended on the activity of the structurally and functionally dissimilar foreign carboxylase. As a further consequence of this mutagenic replacement, the mutant also lacked microscopically observable carboxysomes, the subcellular inclusion bodies in which the wild-type carboxylase naturally resides. The mutant, dependent on a carboxylase with an inferior relative specificity for CO2 versus O2 and apparently lacking carboxysomes, is extremely sensitive to the CO2/O2 ratio supplied during growth and is unable to grow at all in air. This response to the gas composition should prove useful for selection of various R. rubrum carboxylase mutants with altered specificities for CO2 and O2.

References

Jan 26, 1976·Biochemical and Biophysical Research Communications·T TakabeT Akazawa
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1972·Archiv für Mikrobiologie·R A PelroyR Y Stanier
Apr 5, 1981·Journal of Molecular Biology·A OkaM Takanami

❮ Previous
Next ❯

Citations

Dec 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J D Palmer
Apr 1, 1988·Photosynthesis Research·J Pierce, T Omata
Mar 10, 2006·Applied Microbiology and Biotechnology·Thomas A Bobik
Aug 1, 2007·Photosynthesis Research·Archie R Portis, Martin A J Parry
Jul 16, 2008·Photosynthesis Research·Oliver Mueller-Cajar, Spencer M Whitney
May 11, 2011·Photosynthesis Research·George S Espie, Matthew S Kimber
Jun 19, 2013·Photosynthesis Research·James V MoroneyDavid J Longstreth
Jun 5, 2003·Archives of Biochemistry and Biophysics·T John Andrews, Spencer M Whitney
Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert J Spreitzer
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yonatan SavirTsvi Tlusty
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y TakeshimaH Hagiwara
Jan 25, 2013·Journal of Experimental Botany·Moritz Meyer, Howard Griffiths
Jan 21, 2006·Protein Engineering, Design & Selection : PEDS·Monal R ParikhIchiro Matsumura
Dec 30, 1999·Journal of Bacteriology·M F Vázquez-BermúdezE Flores
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Aaron Kaplan, Leonora Reinhold
Sep 12, 2002·Annual Review of Plant Biology·Robert J Spreitzer, Michael E Salvucci
Jan 1, 1996·Annual Review of Microbiology·J R RothT A Bobik
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M Whitney, T J Andrews
Mar 19, 2016·The Plant Journal : for Cell and Molecular Biology·C Raul Gonzalez-EsquerCheryl A Kerfeld
Sep 1, 2017·FEMS Microbiology Letters·Di LiuOliver Mueller-Cajar
Apr 10, 2016·Current Opinion in Plant Biology·Cheryl A Kerfeld, Matthew R Melnicki
Apr 3, 2021·Current Opinion in Microbiology·Julia S Borden, David F Savage

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.