A cyclic AMP protein kinase A-dependent mechanism by which rotavirus impairs the expression and enzyme activity of brush border-associated sucrase-isomaltase in differentiated intestinal Caco-2 cells

Cellular Microbiology
Sandra Martin-LatilA Servin

Abstract

We undertook a study of the mechanism by which rhesus monkey rotavirus (RRV) impairs the expression and enzyme activity of brush border-associated sucrase isomaltase (SI) in cultured, human, fully differentiated, intestinal Caco-2 cells. We provide evidence that the RRV-induced defects in the expression and enzyme activity of SI are not related to the previously observed, RRV-induced, Ca2+ -dependent, disassembly of the F-actin cytoskeleton. This conclusion is based on the facts that: (i) the intracellular Ca2+ blocker, BAPTA/AM, which antagonizes the RRV-induced increase in [Ca2+](i), fails to inhibit the RRV-induced decrease in SI expression and enzyme activity; and (ii) Jasplakinolide (JAS) treatment, known to stabilize actin filaments, had no effect on the RRV-induced decrease in SI expression. Results reported here demonstrate that the RRV-induced impairment in the expression and enzyme activity of brush border-associated SI results from a hitherto unknown mechanism involving PKA signalling. This conclusion is based on the observations that (i) intracellular cAMP was increased in RRV-infected cells and (ii) treatment of RRV-infected cells with PKA blockers resulted in the reappearance of apical SI expression, accompanied b...Continue Reading

References

Jan 1, 1992·Current Topics in Developmental Biology·M B Heintzelman, M S Mooseker
Feb 13, 1990·Biochemistry·P L YeagleB S Eckert
Jan 1, 1991·Annual Review of Cell Biology·A Bretscher
Oct 1, 1990·The Journal of Cell Biology·A Le BivicE Rodriguez-Boulan
Mar 1, 1966·Analytical Biochemistry·M Messer, A Dahlqvist
Nov 18, 1980·Biochimica Et Biophysica Acta·H HauserD E Bowyer
Feb 12, 1998·The American Journal of Physiology·B M Denker, S K Nigam
Dec 4, 1998·Seminars in Cell & Developmental Biology·E Ikonen, K Simons
Feb 5, 2000·Current Biology : CB·K Matter
Jun 22, 2000·Skin Pharmacology and Applied Skin Physiology·T TakahashiY Yokoo
Sep 1, 2000·American Journal of Physiology. Renal Physiology·E J WeinmanS Shenolikar
Sep 26, 2000·Alcoholism, Clinical and Experimental Research·G Negron, B S Eckert
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·K G DickmanR D Shaw
Nov 22, 2000·The Journal of Cell Biology·A ZahraouiT Galli
May 1, 2001·Journal of Molecular Biology·A FelicielloE V Avvedimento
Jul 28, 2001·Kidney International·E J WeinmanS Shenolikar
Aug 10, 2001·Current Opinion in Microbiology·M Ciarlet, M K Estes

❮ Previous
Next ❯

Citations

Jan 29, 2009·The Veterinary Clinics of North America. Food Animal Practice·D M Foster, Geof W Smith
Dec 14, 2011·The Journal of General Virology·Victoria SherwoodKenneth H Mellits
Sep 18, 2015·Advances in Virology·Judith M BallRebecca D Parr
Feb 1, 2005·Virology·Charlotte ChaïbiMonique Géniteau-Legendre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.